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Viruses associated with measles-like illnesses in Uganda
Prossy Namuwulya1, Shirin Ashraf2, Marc Niebel2
1Uganda Virus Research Institute (UVRI), Entebbe, Uganda.
The Journal of Infection
|April 8, 2024
Summary
Metagenomic sequencing identified diverse viruses causing measles-like illnesses in Uganda, including vaccine-preventable ones. This highlights the need for advanced diagnostics and vaccination strategies in the region.
Area of Science:
- Virology
- Infectious Diseases
- Public Health
Background:
- Measles-like illnesses (MLI) pose a diagnostic challenge in Uganda.
- Understanding the causative agents of MLI is crucial for effective public health interventions.
Purpose of the Study:
- To investigate the viral etiology of MLI in Uganda.
- To inform diagnostic assay selection and vaccination strategies.
Main Methods:
- Metagenomic next-generation sequencing (M-NGS) was employed on patient samples from 2010-2019.
- Samples were collected from individuals with MLI who tested IgM negative for measles.
Main Results:
- Viral genomes were detected in 32% of samples.
- 16% of samples contained known viral pathogens, including rubella, parvovirus B19, Epstein-Barr virus, and cytomegalovirus.
- Novel viruses such as Saffold virus and human adenovirus C2 were also identified.
Conclusions:
- Undiagnosed viral infections, including vaccine-preventable illnesses, are a significant cause of MLI in Uganda.
- NGS is a valuable tool for monitoring viral infections in low and middle-income countries.
- Findings can guide vaccination policy and optimize diagnostic approaches.
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