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Coronavirus

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...

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Related Experiment Video

Updated: Jun 17, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Accelerated Development of a COVID-19 Lateral Flow Test in an Academic Setting: Lessons Learned.

Katerina Kourentzi¹, Kristen Brosamer², Binh Vu¹

¹William A. Brookshire Department of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas 77004, United States.

Accounts of Chemical Research

|April 9, 2024

Summary

This summary is machine-generated.

Researchers developed a sensitive, low-cost lateral flow assay (LFA) using glow stick chemistry for COVID-19 antigen detection. This novel approach offers a promising, user-friendly point-of-care diagnostic solution.

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Last Updated: Jun 17, 2026

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Area of Science:

Biomedical Engineering
Diagnostic Technologies
Point-of-Care Testing

Background:

The COVID-19 pandemic highlighted the need for accessible, reliable point-of-care diagnostics, especially for home self-testing.
Traditional antigen tests often balance sensitivity with reader complexity; higher-analytical-sensitivity reporter technologies are crucial for lateral flow assays (LFAs).

Purpose of the Study:

To develop a sensitive, inexpensive, and deployable lateral flow assay (LFA) for SARS-CoV-2 nucleoprotein detection.
To adapt glow stick peroxyoxalate chemistry for a novel light-emitting reporter technology in LFAs.

Main Methods:

Screened over 250 antibody pairs for analytical sensitivity and specificity in LFA format.
Optimized LFA components, including membranes, swabs, extraction reagents, and conjugate preparation.
Developed a smartphone-based reader with a custom app and optimized excitation liquid packaging.

Main Results:

Achieved strong proof-of-concept for a glow stick-inspired light-emitting reporter LFA.
Demonstrated excellent preclinical performance with contrived and clinical samples.
Developed a functional prototype reader usable with a smartphone and a simple dark box.

Conclusions:

The developed LFA platform shows significant promise for sensitive, low-cost antigen detection at the point-of-care.
While commercialization was not achieved within the project timeline, the platform advanced translational aspects and demonstrated feasibility.
The study provides a workflow to guide others in the rapid development of translatable LFAs.