Integrated analysis of lactate-related genes identifies POLRMT as a novel marker promoting the proliferation, migration and energy metabolism of hepatocellular carcinoma via Wnt/β-Catenin signaling
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View abstract on PubMed
Summary
This summary is machine-generated.Researchers identified key lactate-related genes (LRGs) to predict hepatocellular carcinoma (HCC) patient survival. A novel prognostic signature, including POLRMT, shows potential for assessing HCC prognosis and offers new therapeutic targets.
Area Of Science
- Oncology
- Molecular Biology
- Cancer Metabolism
Background
- Hepatocellular carcinoma (HCC) is a leading cause of cancer death globally with poor outcomes.
- Lactate is increasingly recognized for its role in tumor progression, metabolism, and treatment response.
- The specific contribution of lactate-related genes (LRGs) to HCC development and prognosis remains largely undefined.
Purpose Of The Study
- To investigate the role of LRGs in HCC progression and patient survival.
- To develop a prognostic signature for HCC based on LRGs.
- To explore the functional role of specific LRGs, such as POLRMT, in HCC.
Main Methods
- Analysis of The Cancer Genome Atlas (TCGA) datasets to identify differentially expressed LRGs.
- Pan-cancer analysis to assess LRG expression across various tumor types.
- Development of a prognostic signature using LASSO-Cox regression and validation through survival analysis.
- Gene Set Variation Analysis (GSVA) to correlate gene expression with clinical and immune characteristics.
- Functional assays to determine the role of POLRMT in HCC cell proliferation, migration, and metabolism.
Main Results
- Identified 21 differentially expressed LRGs associated with long-term survival in HCC patients.
- These 21 LRGs classified HCC into two distinct molecular subtypes with significant differences in clinical and immune profiles.
- A novel prognostic signature comprising nine LRGs (DTYMK, IRAK1, POLRMT, MPV17, UQCRH, PDSS1, SLC16A3, SPP1, and LDHD) accurately predicted HCC patient survival.
- High expression of POLRMT was observed in HCC tissues and cell lines, promoting proliferation, migration, and energy metabolism via the Wnt/β-Catenin pathway.
Conclusions
- The developed prognostic signature offers potential clinical value for assessing HCC patient prognosis.
- POLRMT is identified as a key oncogene in HCC, promoting tumor progression and serving as a potential therapeutic target.
- These findings enhance the understanding of HCC pathophysiology and contribute to the development of novel therapeutic strategies.
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