Effect of microRNA-663b on migration, invasion and epithelial‑mesenchymal transition of oral squamous cell carcinoma...

To explore the effect of microRNA-663b (miR-6636) on migration, invasion and epithelial-mesenchymal transition (EMT) of oral squamous cell carcinoma cells (OSCC).

Use R Studio of gene expression omnibus (GEO) database to analyze expressions of miR-663b in the OSCC and adjacent normal tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-663b in tissues and cells. The transfection efficiency of HN30 cells with miR-663b knockout plasmid was detected. Transwell assay was used to detect the effect of the migration and invasion ability. Bioinformatics method was used to predict the targeted mRNA that may bind to miR-663b and double luciferase assay was used to verify the binding. Western blot assay was used to detect the expression of EMT-related markers.

The expression of miR-663b was up-regulated in OSCC tissues and higher in HN30, CAL27 and SCC-9 cells than in HOEC cells (P<0.05). Knockout of miR-663b could inhibit migration and invasion of HN30 cells (P<0.05) and inhibit the occurrence of EMT. Bioinformatics prediction software predicts that SH3BP2 was the target gene of miR-663b, and patients with low SH3BP2 expression had a poor prognosis (P<0.05). MiR-663b could bind to SHBP2 (P<0.05). The expression of SH3BP2 was increased and the occurrence of EMT was inhibited in HN30 cells with miR-663b knocked out.

Knockout of miR-663b can inhibit the migration, invasion and EMT of OSCC by targeting SH3BP2.