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Updated: Jun 28, 2025

09:43
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
Published on: October 31, 2013
13.5K
The single strand template shortening strategy improves the sensitivity and specificity of solid-state nanopore
Abstract:
Through controlling the ssDNA product length of rolling circle amplification with AcyNTP, here we develop a nanopore signal enhancement strategy (STSS), which can successfully transfer the short oligonucleotide targets into long ssDNAs with appropriate lengths that can generate significant translocation currents. By labelling the RCA product with tags such as tetrahedral structures and isothermal amplicons, the resolution, signal specificity, and target range of the STSS can be further extended.
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