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Updated: Jun 28, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Description of Corynebacterium hiratae sp. nov. isolated from a human tissue bone a novel member of Corynebacterium
Lincoln de Oliveira Sant'Anna1, Louisy Sanches Dos Santos2, Julianna Giordano Botelho Olivella2
1Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, State University of Rio de Janeiro, Vinte e Oito de Setembro 87, Fundos, 3º andar. Vila Isabel, Rio de Janeiro, RJ, CEP:20551-030, Brazil. lincolnsantanna2013@gmail.com.
Background:
Corynebacterium spp. are widely disseminated in the environment, and they are part of the skin and mucosal microbiota of animals and humans. Reports of human infections by Corynebacterium spp. have increased considerably in recent years and the appearance of multidrug resistant isolates around the world has drawn attention.
Objectives:
To describe a new species of Corynebacterium from human tissue bone is described after being misidentified using available methods.
Methods:
For taxonomic analyses, phylogenetic analysis of 16S rRNA and rpoB genes, in silico DNA-DNA hybridization, average nucleotide and amino acid identity, multilocus sequence analysis, and phylogenetic analysis based on the complete genome were used.
Findings:
Genomic taxonomic analyzes revealed values of in silico DNA-DNA hybridization, average nucleotide and amino acids identity below the values necessary for species characterization between the analyzed isolates and the closest phylogenetic relative Corynebacterium aurimucosum DSM 44532T.
Main Conclusions:
Genomic taxonomic analyzes indicate that the isolates analyzed comprise a new species of the Corynebacterium genus, which we propose to name Corynebacterium hiratae sp. nov. with isolate 332T (= CBAS 826T = CCBH 35,014T) as the type strain.
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