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Related Experiment Videos

Cell-cycle-specific genes differentially expressed in human leukemias.

B Calabretta, L Kaczmarek, W Mars

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1985
    PubMed
    Summary

    Cell cycle gene expression in leukemia reveals potential new oncogenes. These genes, preferentially active in G1 phase, show altered expression patterns in various leukemias, suggesting a role in malignancy.

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    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Oncology

    Background:

    • Cell cycle regulation is crucial for normal cell proliferation.
    • Dysregulation of cell cycle genes is implicated in cancer development.
    • Specific cell cycle genes (p4F1, p2F1, p2A9) were identified in hamster cells.

    Purpose of the Study:

    • To investigate the cell cycle-dependent expression of hamster cDNA clones (p4F1, p2F1, p2A9) in human cells.
    • To analyze the expression patterns of these genes in normal human lymphocytes and various leukemia types.
    • To explore the potential role of cell cycle genes in human malignancies.

    Main Methods:

    • Isolation of three cDNA clones (p4F1, p2F1, p2A9) from Syrian hamster cells.
    • Analysis of gene expression in human peripheral blood mononuclear cells stimulated with phytohemagglutinin.

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  • Investigation of gene expression in human diploid fibroblasts (WI-38).
  • Quantitative assessment of gene expression levels in different types of human leukemia (lymphoid and myeloid).

    • Main Results:

    • p4F1 and p2F1 expression is cell cycle-dependent in phytohemagglutinin-stimulated human lymphocytes.
    • p2A9 expression is cell cycle-dependent in human diploid fibroblasts but not clearly detected in lymphocytes.
    • Differential expression levels of p2A9, p2F1, and p4F1 were observed across various leukemia subtypes.
    • p2A9 showed highest expression in chronic myelogenous leukemia and lowest in chronic lymphocytic leukemia.
    • p2F1 and p4F1 exhibited highest expression in chronic and acute myelogenous leukemia.
    • Two other cell-cycle genes were not detectable in human leukemias.

    Conclusions:

    • Cell cycle-dependent genes exhibit distinct expression patterns in human leukemias.
    • Aberrant expression of cell division cycle genes may contribute to the phenotype of human malignancies.
    • These findings suggest that cell cycle-regulated genes could represent novel oncogenes.