Microbe cultivation guidelines to optimize rhamnolipid applications

In the growing landscape of interest in natural surfactants, selecting the appropriate one for specific applications remains challenging. The extensive, yet often unsystematized, knowledge of microbial surfactants, predominantly represented by rhamnolipids (RLs), typically does not translate beyond the conditions presented in scientific publications. This limitation stems from the numerous variables and their interdependencies that characterize microbial surfactant production. We hypothesized that a computational recipe for biosynthesizing RLs with targeted applicational properties could be developed from existing literature and experimental data. We amassed literature data on RL biosynthesis and micellar solubilization and augmented it with our experimental results on the solubilization of triglycerides (TGs), a topic underrepresented in current literature. Utilizing this data, we constructed mathematical models that can predict RL characteristics and solubilization efficiency, represented as logP_RL = f(carbon and nitrogen source, parameters of biosynthesis) and logMSR = f(solubilizate, rhamnolipid (e.g. logP_RL), parameters of solubilization), respectively. The models, characterized by robust R² values of respectively 0.581-0.997 and 0.804, enabled the ranking of descriptors based on their significance and impact-positive or negative-on the predicted values. These models have been translated into ready-to-use calculators, tools designed to streamline the selection process for identifying a biosurfactant optimally suited for intended applications.