Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

3.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.6K
Cohesins02:20

Cohesins

4.5K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.5K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.2K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perioperative and post-hospital whole-course nutrition management in patients with pancreatoduodenectomy - a single-center prospective randomized controlled trial.

International journal of surgery (London, England)·2024
Same author

Blood-Brain Barrier Penetrating Nanovehicles for Interfering with Mitochondrial Electron Flow in Glioblastoma.

ACS nano·2024
Same author

Ill-fitting prosthesis is associated with an increased risk of elevated blood pressures.

Journal of oral rehabilitation·2024
Same author

Melatonin improves salivary gland damage and hypofunction in pSS by inhibiting IL-6/STAT3 signaling through its receptor-dependent manner.

Molecular immunology·2024
Same author

ALK upregulates POSTN and WNT signaling to drive neuroblastoma.

Cell reports·2024
Same author

CellVisioner: A Generalizable Cell Virtual Staining Toolbox based on Few-Shot Transfer Learning for Mechanobiological Analysis.

Research (Washington, D.C.)·2024

Related Experiment Video

Updated: Jun 28, 2025

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.6K

CBX7C⋅PHC2 interaction facilitates PRC1 assembly and modulates its phase separation properties.

Shanli Guan1, Jiajia Tang1, Xiaojun Ma1

  • 1School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu, P.R. China.

Iscience
|April 11, 2024
PubMed
Summary

CBX7C, a novel protein in mouse embryonic stem cells, acts as an epigenetic repressor. Its interaction with PHC2 is crucial for Polycomb Repressive Complex 1 (PRC1) assembly and cell differentiation.

Keywords:
Cell biologyEpigeneticsMolecular biology

More Related Videos

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

11.3K
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

3.4K

Related Experiment Videos

Last Updated: Jun 28, 2025

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.6K
In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

11.3K
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

3.4K

Area of Science:

  • Epigenetics
  • Stem cell biology
  • Molecular and cell biology

Background:

  • CBX7 is a core component of the Polycomb Repressive Complex 1 (PRC1).
  • Cbx7C is a previously uncharacterized splicing isoform of CBX7, specifically found in mouse embryonic stem cells (mESCs).

Purpose of the Study:

  • To investigate the function of the CBX7C isoform in mESCs.
  • To elucidate the role of CBX7C in PRC1 assembly and function.
  • To understand the impact of CBX7C on mESC differentiation and Polycomb body formation.

Main Methods:

  • Studied the interaction between CBX7C and PHC2.
  • Analyzed CBX7C and PHC2 expression during mESC differentiation.
  • Performed knockdown experiments of Cbx7C in mESCs.
  • Investigated the formation and properties of Polycomb bodies under varying CBX7C and PHC2 concentrations.

Main Results:

  • CBX7C functions as an epigenetic repressor at PRC1 target sites in mESCs.
  • CBX7C's interaction with PHC2 facilitates PRC1 complex assembly.
  • Both Cbx7C and Phc2 are upregulated during mESC differentiation, and Cbx7C knockdown inhibits this process.
  • Low CBX7C⋅PHC2 levels promote functional, mobile Polycomb bodies, while high levels lead to large, immobile, chromatin-free aggregates.

Conclusions:

  • CBX7C plays a unique role in PRC1 assembly and Polycomb body formation.
  • The CBX7C⋅PHC2 interaction's phase separation properties can be modulated to control PRC1 activity.
  • Findings offer new strategies for manipulating PRC1 functions through phase separation control.