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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
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Retrovirus Life Cycles01:10

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Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
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  9. Predictive And Prognostic Markers
  11. Hbo1/kat7/myst2 Hat Complex Regulates Human Adenovirus Replicative Cycle.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Hbo1/kat7/myst2 Hat Complex Regulates Human Adenovirus Replicative Cycle.

Related Experiment Video

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

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HBO1/KAT7/MYST2 HAT complex regulates human adenovirus replicative cycle.

Heba Kamel1, Varsha Shete1, Sayikrushna Gadamsetty1

  • 1Microbiology and Immunology Department, Fienberg School of Medicine, Northwestern University, Chicago, USA.

Heliyon
|April 11, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Human adenoviruses (HAdV) recruit the HBO1 histone acetyltransferase complex for replication. Downregulating HBO1 reduces viral gene expression and HAdV5 growth by affecting histone acetylation at viral promoters.

Keywords:
Adenovirus type 5ChromatinE1AHAT

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Related Experiment Videos

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Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments
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Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments

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Area of Science:

  • Virology
  • Molecular Biology
  • Epigenetics

Background:

  • Human adenoviruses (HAdV) are DNA tumor viruses that utilize cellular machinery for replication.
  • Understanding viral hijacking of host cell processes is key to virology research.
  • HBO1 (Histone Acetyltransferase Binding to ORC1) is a histone acetyltransferase complex involved in cell proliferation.

Purpose of the Study:

  • To investigate the role of HBO1 in human adenovirus type 5 (HAdV5) replication.
  • To determine if HBO1 influences viral gene expression and histone acetylation during infection.

Main Methods:

  • Downregulation of HBO1 in human cells followed by HAdV5 infection.
  • Analysis of viral early gene expression (e.g., E1A).
  • Assessment of H3 lysine 14 acetylation at viral promoters using ChIP.
HBO1
Viral replication
  • Detection of HBO1 association with viral promoters and replication centers.
  • Co-immunoprecipitation assays to study E1A and HBO1 interaction.

    • Main Results:

    • HBO1 downregulation significantly reduced HAdV5 growth and early viral gene expression.
    • Reduced H3 lysine 14 acetylation at viral promoters was observed in HBO1-downregulated cells.
    • HBO1 was found associated with viral promoters and co-localized with viral replication centers.
    • Overexpression of E1A stimulated HBO1 activity and E1A co-immunoprecipitated with HBO1.

    Conclusions:

    • HAdV5 actively recruits the HBO1 HAT complex to facilitate viral replication.
    • HBO1 plays a critical role in promoting viral gene expression through histone acetylation at viral promoters.
    • The interaction between HAdV and HBO1 highlights a novel mechanism of viral exploitation of host epigenetic machinery.