Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Receptor affinity chromatography.

R A Kohanski, M D Lane

    Annals of the New York Academy of Sciences
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Researchers purified insulin receptors from mouse adipocytes using a novel biotinylated insulin ligand. This method efficiently isolates the receptor for further study.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Linkages between mineralogy, fluid chemistry, and microbial communities within hydrothermal chimneys from the Endeavour Segment, Juan de Fuca Ridge.

    Geochemistry, geophysics, geosystems : G(3)·2018
    Same author

    Mode of action of antibiotics in swine nutrition. I. Effect of feeding antibiotics on blood glucose.

    Antibiotics & chemotherapy (Northfield, Ill.)·2014
    Same author

    Mode of action of antibiotics in swine nutrition. II. Effect of antibiotics on intestinal flora.

    Antibiotics & chemotherapy (Northfield, Ill.)·2014
    Same author

    Studies on the function of vitamin A in metabolism by the use of radioactive metabolic intermediates.

    Progress in nuclear energy. Series 6 Biological sciences·2014
    Same author

    Regulation of food intake and energy expenditure by hypothalamic malonyl-CoA.

    International journal of obesity (2005)·2008
    Same author

    Role of malonyl-CoA in the hypothalamic control of food intake and energy expenditure.

    Biochemical Society transactions·2005
    Same journal

    Multiomics Profiling During Autoimmune Demyelination Highlights a Complex Regulatory Role for Ataxin-1 in B Cells.

    Annals of the New York Academy of Sciences·2026
    Same journal

    Global Trends in Light Pollution and Their Relationship With Socioeconomic Factors.

    Annals of the New York Academy of Sciences·2026
    Same journal

    Wired for Corruption: Inter-Brain Synchrony Encodes Bribery-Related Value Information and Predicts Bribery Agreement.

    Annals of the New York Academy of Sciences·2026
    Same journal

    LM-YOLO: A Lightweight Multi-Scale Enhanced Model for Forest Smoke Detection Using Unmanned Aerial Vehicles.

    Annals of the New York Academy of Sciences·2026
    Same journal

    Polyrhythm Perception and Production: A Scoping Review.

    Annals of the New York Academy of Sciences·2026
    Same journal

    DARTS-CNN-BiLSTM: Intelligent Fault Diagnosis for Computer Numerical Control Machine Tool Feed System.

    Annals of the New York Academy of Sciences·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Background:

    • The insulin receptor plays a crucial role in glucose metabolism and cellular signaling.
    • Efficient purification of the insulin receptor is essential for detailed biochemical and structural studies.

    Purpose of the Study:

    • To develop a high-yield purification method for the insulin receptor from cultured 3T3-L1 mouse adipocytes.
    • To utilize a novel bifunctional ligand for affinity purification.

    Main Methods:

    • Purification of insulin receptor using a bifunctional ligand: N alpha B1-(biotinyl-epsilon-aminocaproyl)insulin.
    • Employing avidin-Sepharose CL-4B affinity chromatography for receptor isolation.
    • Removal of endogenous biotin-binding proteins using native avidin-Sepharose.

    Related Experiment Videos

  • Elution of the purified insulin receptor with biotin.

    • Main Results:

    • High yield purification of insulin receptor achieved from 3T3-L1 mouse adipocytes.
    • The bifunctional ligand demonstrated high competence for both insulin and biotin binding.
    • The avidin-Sepharose matrix showed efficient biotin binding and regenerability.
    • Biotin successfully displaced the purified insulin receptor from the affinity matrix.

    Conclusions:

    • The developed method provides an effective means for high-yield insulin receptor purification.
    • This technique facilitates further investigation into insulin receptor function and structure.
    • The bifunctional ligand approach offers a robust strategy for purifying specific receptor proteins.