Related Experiment Video
Updated: May 5, 2026

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
Açaí Flan, A Functional Food with Lacticaseibacillus rhamnosus HN001 Probiotic: Physicochemical Characteristics,
Paulyne Tolentino Anselmo1, Beatriz Cardoso Sabino1, Carla Prado Rosolém1
1Universidade Pitágoras Unopar, Programa de Mestrado em Ciência e Tecnologia de Leite e Derivados, Marselha street, 183, Zip code: 86041-140 Londrina, PR, Brazil.
Research Background:
Açaí berry is rich in antioxidant compounds and is therefore closely associated with beneficial health effects. In this study, we aim to investigate the potential of using Lacticaseibacillus rhamnosus HN001 as a probiotic culture on açaí flan.
Experimental Approach:
The chemical composition, physicochemical and microbiological characteristics, and sensory acceptance during refrigerated storage (5 °C for 42 days) of flan were investigated. In addition, the consumer perception of the product was evaluated using word association when consumers were shown a photo of the product with or without the added ingredients accompanied with a brief description of the product.
Results And Conclusions:
The flan had a suitable chemical composition, mainly carbohydrates and proteins, probiotic viability reached 8 log CFU/g in the product and 4 log CFU/g after gastrointestinal simulation, typical açaí coloration, significant antioxidant activity and high sensory acceptability. The information about the ingredients and properties of the products increased the health value and positive feelings of the consumers towards the product.
Novelty And Scientific Contribution:
Açaí flan has proven to be a suitable carrier for L. rhamnosus HN001 as a probiotic culture, further enhancing the characteristic beneficial properties of the fruit. Therefore, combining this information with marketing strategies that inform consumers about the benefits of the product can further improve its acceptance. As far as we know, this is the first study on açaí flan with added probiotic culture.
More Related Videos
Related Concept Videos
Microorganisms in Agriculture and Food industry
Microbes in Food Production
Microbes in the Production of Fermented Foods
Probiotics
Microbial Spoilage of Food
Production of Organic Acids

