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Biosensing with Oleosin-Stabilized Liquid Crystal Droplets.

Lawrence W Honaker1, Axel Eijffius1, Lorenz Plankensteiner2,3

  • 1Laboratory of Physical Chemistry and Soft Matter, Wageningen University & Research, 6708 WE, Wageningen, The Netherlands.

Small (Weinheim an Der Bergstrasse, Germany)
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Summary

Oleosins stabilize liquid crystal (LC) droplets, enabling sensitive detection of surfactants. This biocompatible approach offers a new platform for biosensing applications using plant-derived proteins.

Keywords:
amphipathic helixbiosensingliquid crystaloleosinstructural color

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biochemistry

Background:

  • Liquid crystals (LCs) are versatile platforms for chemical, physical, and biological sensing.
  • Existing LC-based sensors face challenges in maintaining sensitivity, responsiveness, and biocompatibility for detecting biological amphiphiles.

Purpose of the Study:

  • To investigate the stabilization of LC droplets using oleosins, surface-active plant proteins.
  • To evaluate the performance of oleosin-stabilized LC droplets as biosensors for amphiphiles.

Main Methods:

  • Stabilization of nematic and chiral LC droplets using purified oleosins at sub-micromolar concentrations.
  • Fluorescent labeling to confirm oleosin localization at the LC-water interface.
  • Assessment of surfactant detection sensitivity and stability of the LC droplets.

Main Results:

  • Oleosins effectively stabilized nematic LC droplets at sub-micromolar concentrations without altering alignment, enabling micromolar surfactant detection.
  • Oleosin localization at the LC-water interface was confirmed via fluorescent labeling.
  • Chiral LC droplets exhibited a sensitive response to nanomolar oleosin concentrations, indicating potential for highly sensitive amphipathic helix detection.

Conclusions:

  • Oleosin-stabilized nematic LC droplets provide a biocompatible and stable platform for bioanalyte detection.
  • Chiral LC systems show promise as highly sensitive sensors for biological amphipathic helices.