Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Adipocyte-Derived Leptolin Enhances Energy Expenditure and Prevents Obesity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Global trends, epidemiological patterns, and disability burden of metabolic dysfunction-associated steatotic liver disease-related cirrhosis from 1990 to 2021: A comprehensive analysis of the Global Burden of Disease study.

The Journal of international medical research·2026

Same author

A small-molecule stabilizer of the calpastatin-calpain-2 complex restores mitochondrial function and mitigates neurodegeneration.

Science advances·2026

Same author

Strategic modification of outer branched side chains in multi-modal phototheranostic nanoplatform for synergistic breast cancer therapy.

Journal of colloid and interface science·2026

Same author

Ephrin and Eph family proteins: Molecular mechanisms of signaling pathways and their role in neural injury repair.

Neural regeneration research·2026

Same author

Bacteria and phage consortia modulate cecal SCFA production and host metabolism to enhance feed efficiency in ducks.

Microbiome·2026

Search research articles

Related Experiment Video

Updated: Jun 28, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

Proteomics Platform Reveals Broad-Spectrum Nanobodies for SARS-CoV-2 Variant Neutralization.

Ran Zhang^1,2, Lan Huang³, Xiaohan Zhang⁴

¹School of Basic Medicine Sciences, Anhui Medical University, Hefei, Anhui 230031, PR China.

Journal of Proteome Research

|April 11, 2024

Summary

Researchers developed a high-throughput proteomics platform to screen nanobodies against SARS-CoV-2 variants. Two nanobodies, C-282 and C-39, showed broad neutralizing activity against multiple variants, offering potential future treatments.

Keywords:

SARS-CoV-2 microarray nanobody neutralization proteomics

More Related Videos

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

Related Experiment Videos

Last Updated: Jun 28, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

Area of Science:

Virology and Immunology
Biotechnology and Proteomics

Background:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants exhibit immune evasion, necessitating novel therapeutic strategies.
Nanobodies offer advantages like small size, stability, and high affinity for developing neutralizing antibodies.

Purpose of the Study:

To establish an integrated proteomics platform for high-throughput screening of nanobodies against SARS-CoV-2 spike variants.
To identify and characterize nanobodies with broad neutralizing activity against diverse SARS-CoV-2 variants.

Main Methods:

Immunization of a camel with wild-type SARS-CoV-2 spike protein (S1 subunit) and construction of a nanobody phage library.
High-throughput screening of the nanobody library against 72 SARS-CoV-2 spike variants.
In vitro pseudovirus-based neutralization assays to validate nanobody efficacy.

Main Results:

Identification of two nanobodies, C-282 and C-39, demonstrating broad neutralizing activity.
These nanobodies effectively neutralized six non-Omicron variants (D614G, Alpha, Beta, Gamma, Delta, Kappa) and five Omicron variants (BA.1-5).

Conclusions:

The developed proteomics platform is effective for identifying nanobodies with broad neutralizing capabilities against SARS-CoV-2 variants.
Identified nanobodies hold potential for future therapeutic development against SARS-CoV-2 infections.