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Oncology And Carcinogenesis
Predictive And Prognostic Markers
Identification And Validation Of Anoikis-related Genes To Clarify The Prognosis And Immune Mechanisms Of Patients With Low-grade Glioma.

Home
Research Domains
Biomedical And Clinical Sciences
Oncology And Carcinogenesis
Predictive And Prognostic Markers
Identification And Validation Of Anoikis-related Genes To Clarify The Prognosis And Immune Mechanisms Of Patients With Low-grade Glioma.

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Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

Identification and validation of anoikis-related genes to clarify the prognosis and immune mechanisms of patients with low-grade glioma.

Ji Wang¹, Yantin Liu¹, Min Qian²

¹Institute of Neurology, The First College of Clinical Medical Science, China Three Gorges University, Department of Neurosurgery, Yichang Central People's Hospital, Yichang, 443000, China.

Biochemical and Biophysical Research Communications

|April 11, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Tissue inhibitor of metalloproteinase 1 (TIMP1) promotes low-grade glioma (LGG) malignancy by preventing anoikis, a programmed cell death. This finding offers new therapeutic targets for LGG.

Keywords:

Anoikis Immune infiltration Low-grade glioma Macrophage genes

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Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Low-grade glioma (LGG) presents a poor prognosis, with mechanisms of malignant progression poorly understood.
Anoikis, a form of programmed cell death, and Tissue Inhibitor of Metalloproteinase 1 (TIMP1) are implicated in cancer development.

Purpose of the Study:

To investigate the role and mechanism of anoikis and TIMP1 in the malignant progression of low-grade glioma.
To develop a prognostic model for LGG based on anoikis-related genes.

Main Methods:

Screened seven anoikis-related genes from TCGA and GEO databases to build a prognostic model.
Assessed clinical prognosis, pathological characteristics, and immune cell infiltration in high- and low-risk groups.
Investigated TIMP1's effects on LGG proliferation, migration, and anoikis in vitro and in vivo.

Single-cell RNA sequencing

Main Results:

Identified seven key genes, including PTGS2, CCND1, TIMP1, PDK4, LGALS3, CDKN1A, and CDKN2A.
Kaplan-Meier curves showed significant correlations between clinical features and overall survival.
TIMP1 was found to promote LGG malignant progression by inhibiting anoikis.

Conclusions:

TIMP1 promotes LGG malignant progression through anoikis inhibition, revealing potential therapeutic strategies.
Findings provide insights into LGG pathogenesis and identify TIMP1 as a potential therapeutic target.