Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Democratized single-cell proteomics resolves cell state heterogeneity in skin tumors.

Life science alliance·2026
Same author

Roles of PknB and CslA in Cell Wall Morphogenesis of Streptomyces.

Molecular microbiology·2026
Same author

Global identification of Chromobacterium violaceum T6SS effectors reveals an Rhs antibacterial toxin featuring FIX and ADP-ribosyltransferase domains.

The Journal of biological chemistry·2026
Same author

Targeting noncanonical nuclear factor kappa B signalling in CYLD cutaneous syndrome by selective inhibition of IκB kinase alpha.

The British journal of dermatology·2026
Same author

Optimising the induction of inflammation within preterm infant-derived intestinal epithelial organoids.

Npj gut and liver·2026
Same author

A proteomics investigation of primary human articular chondrocyte isolation.

Osteoarthritis and cartilage open·2025

Related Experiment Video

Updated: Jun 28, 2025

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
08:04

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

12.3K

Stability-based approaches in chemoproteomics.

Amy L George1, Maria Emilia Dueñas1, José Luis Marín-Rubio1

  • 1Laboratory for Biomedical Mass Spectrometry, Biosciences Institute, Newcastle University, Newcastle-upon-Tyne, NE2 4HH, UK.

Expert Reviews in Molecular Medicine
|April 11, 2024
PubMed
Summary
This summary is machine-generated.

Chemoproteomics advances target deconvolution for drug discovery. Modification-free methods analyzing protein thermodynamics offer proteome-wide insights, accelerating the development of safer and more effective therapeutics.

Keywords:
chemical proteomicschemoproteomicsdrug discoverylimited proteolysismass spectrometryproteome integral solubility alterationtarget deconvolutiontarget engagementthermal proteome profiling

More Related Videos

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.0K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

Related Experiment Videos

Last Updated: Jun 28, 2025

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
08:04

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

12.3K
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.0K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Pharmacology

Background:

  • Target deconvolution is crucial for understanding drug mechanisms and optimizing therapeutic potential.
  • Chemoproteomics, integrating chemical biology and mass spectrometry, has revolutionized target deconvolution.
  • Existing methods often rely on specific protein target enrichment, limiting scope.

Purpose of the Study:

  • To review modification-free chemoproteomic approaches for target deconvolution.
  • To highlight methods leveraging changes in protein thermodynamics upon small molecule binding.
  • To discuss the impact of these advancements on drug discovery and development.

Main Methods:

  • Focus on modification-free chemoproteomic strategies.
  • Leveraging shifts in protein thermodynamics induced by ligand binding.
  • Utilizing advancements in mass spectrometry for proteome-wide analysis.

Main Results:

  • Modification-free approaches enable proteome-wide target deconvolution.
  • These methods are driven by enhanced mass spectrometry sensitivity and quantitation.
  • Advances include thermal/chemical denaturation and protease degradation techniques.

Conclusions:

  • Modification-free chemoproteomics offers comprehensive proteome-wide evaluations.
  • These techniques accelerate the understanding of compound mechanisms of action.
  • The evolving landscape of chemoproteomics significantly impacts future drug development strategies.