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Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR

Xin Zhang1, Yuhan Chen1,2, Tinglu Yan1

  • 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Cell Death Discovery
|April 11, 2024
PubMed

Insights

A novel repressor, CdsR, controls cell death during Bacillus sporulation. It inhibits LrgAB, a protein causing cell lysis, ensuring proper spore development.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Cell Biology

Background:

  • Cell death and survival are critical in biological processes like bacterial spore development.
  • The specific cell death pathways regulating sporulation in Bacillus species are not fully understood.

Purpose of the Study:

  • To identify novel regulators involved in cell death during Bacillus sporulation.
  • To elucidate the mechanism by which cell death is controlled during spore development.

Main Methods:

  • Identification and characterization of a novel ArsR family transcriptional regulator, CdsR.
  • Gene deletion and overexpression studies of cdsR and lrgAB.
  • Analysis of cell lysis and sporulation phenotypes.

Main Results:

  • Deletion of cdsR leads to cell lysis and impaired sporulation in Bacillus.
  • CdsR directly represses the expression of lrgAB.
  • Overexpression of lrgAB causes cell lysis and inhibits sporulation, similar to the cdsR mutant.
  • LrgAB, a holin-like protein, is identified as an inducer of cell death in Bacillus.

Conclusions:

  • A novel ArsR family transcriptional regulator, CdsR, governs cell death in Bacillus.
  • CdsR is essential for preventing LrgAB-mediated cell lysis during sporulation.
  • The study reveals a new regulatory mechanism controlling cell fate in Bacillus species.

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