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CD99 Expression and Prognostic Impact in Glioblastoma: A Single-Center Cohort Study
Andrea Rocca1, Fabiola Giudici2, Carmine Antonio Donofrio3,4
1Department of Medical, Surgical and Health Sciences, University of Trieste, 34147 Trieste, Italy.
Cells
|April 12, 2024
Summary
High expression of CD99 was found in glioblastoma tumors, but this membrane glycoprotein did not significantly impact patient survival. Further research is needed to determine CD99
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cancer Research
Background:
- Glioblastoma is the most aggressive adult brain tumor.
- CD99 is a membrane glycoprotein implicated in cell migration and invasion.
- Understanding CD99's role in glioblastoma is crucial for potential therapeutic strategies.
Purpose of the Study:
- To evaluate CD99 expression in glioblastoma.
- To determine the prognostic impact of CD99 on overall survival (OS).
- To analyze both wild-type (CD99wt) and truncated (CD99sh) isoforms.
Main Methods:
- Retrospective analysis of 46 glioblastoma patients treated with standard therapy.
- Immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR) for CD99 expression.
- Kaplan-Meier, log-rank tests, and multivariable Cox regression for survival analysis.
Main Results:
- CD99 expression was detected in 83% of glioblastomas via IHC.
- Only CD99wt isoform was detected by qRT-PCR, correlating with IHC results (rho = 0.309, p = 0.037).
- CD99 expression did not significantly correlate with OS (p = 0.61 for qRT-PCR, p = 0.73 for IHC) or progression-free survival.
Conclusions:
- Glioblastoma exhibits high CD99 expression.
- CD99 expression does not appear to be a significant prognostic marker for glioblastoma survival.
- Preclinical studies are warranted to elucidate CD99's precise role and therapeutic potential.
Keywords:
CD99glioblastomaimmunohistochemistryprognostic factorquantitative real-time polymerase chain reaction
