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Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

Updated: Jun 20, 2026

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

A Custom Panel for Profiling Microglia Gene Expression.

Phani Sankar Potru¹, Natascha Vidovic¹, Susanne Wiemann¹

¹Bielefeld University, Medical School OWL, Anatomy and Cell Biology, 33615 Bielefeld, Germany.

|April 12, 2024

Summary

This summary is machine-generated.

Researchers developed a 178-gene NanoString panel to quantify microglia gene expression. This cost-effective tool accurately reflects gene changes in various microglia states, aiding neurological disorder research.

Keywords:

BV2 cells NanoString RNA bone marrow monocytes microglia nCounter technology

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Last Updated: Jun 20, 2026

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Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

Area of Science:

Neuroscience
Immunology
Genetics

Background:

Microglia are central nervous system immune cells crucial for development, homeostasis, and neurological health.
Microglia dysfunction is linked to various neurological disorders.
Single-cell studies reveal unique microglia gene expression, necessitating efficient quantification methods.

Purpose of the Study:

To develop a simple, multiplexed approach for quantifying microglia-specific gene expression.
To create a NanoString nCounter-based custom codeset for murine microglia gene analysis.

Main Methods:

Designed a 178-gene murine microglia-specific custom codeset using NanoString nCounter technology.
Analyzed RNA from ex vivo adult mouse microglia, primary mouse microglia, BV2 cell line, and bone marrow monocytes.
Validated the panel using publicly available gene expression datasets.

Main Results:

Homeostatic genes were highly expressed in adult microglia, less in primary cells, and absent in BV2 cells.
Reactive microglia markers were elevated in primary microglia and BV2 cells.
The panel reliably reflected microglia gene expression changes in response to various factors.

Conclusions:

The developed microglia gene expression panel is a swift and cost-effective tool for assessing microglia.
This panel can be utilized to study microglia in diverse contexts, from homeostasis to disease models.