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Updated: Jun 28, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
Published on: February 9, 2024
Bridging biological samples to functional nucleic acid biosensor applications: current enzymatic-based strategies for
David Septian Sumanto Marpaung1, Ayu Oshin Yap Sinaga2, Damayanti Damayanti3
1Department of Biosystems Engineering, Institut Teknologi Sumatera, Jl. Terusan Ryacudu, Way Huwi, Kec. Jati Agung, Lampung Selatan, Lampung, 35365, Indonesia. david.marpaung@tbs.itera.ac.id.
Generating high-quality single-stranded DNA (ssDNA) from biological samples is crucial for advanced biosensor diagnostics. This review details innovative ssDNA production methods to improve disease detection and public health responses.
Area of Science:
- Biotechnology
- Molecular Diagnostics
- Biosensor Technology
Background:
- Emerging diseases necessitate advanced diagnostic tools.
- Functional nucleic acid (FNA) biosensors, utilizing single-stranded DNA (ssDNA), are key for analyte detection.
- Challenges exist in obtaining high-quality ssDNA from biological samples for biosensor integration.
Purpose of the Study:
- To review strategies for generating ssDNA from biological samples.
- To discuss the integration of these ssDNA generation techniques with biosensors.
- To highlight advancements in disease detection and monitoring.
Main Methods:
- Asymmetric PCR
- Exonuclease-PCR
- Isothermal amplification
- Biotin-streptavidin PCR
- Transcription-reverse transcription
- ssDNA overhang generation
- Urea denaturation PAGE
Main Results:
- Various innovative ssDNA generation techniques have been developed.
- These methods enable the production of high-quality ssDNA suitable for biosensor applications.
- Successful integration of ssDNA generation with biosensors for biological sample analysis.
Conclusions:
- Effective ssDNA generation is critical for robust biosensor performance.
- These integrated approaches enhance the speed and accuracy of disease diagnostics.
- Advancements in biotechnology, particularly ssDNA generation for biosensors, strengthen global health security.
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