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Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Optimizing Chromatographic Separations01:15

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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
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Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

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Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
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High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
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Improved assay development of pharmaceutical modalities using feedback-controlled liquid chromatography optimization.

Fatima Naser Aldine1, Andrew N Singh1, Heather Wang1

  • 1Analytical Research and Development, MRL, Merck & Co., Inc., Rahway, NJ 07065, USA.

Journal of Chromatography. A
|April 12, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces an automated analytical workflow that uses artificial intelligence (AI) to streamline chromatographic method development. This AI-driven approach significantly reduces analyst time and accelerates the creation of robust separation assays for biopharmaceutical products.

Keywords:
Achiral separationsAutomated method developmentChiral separationsEnantioseparationFeedback controlled modellingReversed-phase liquid chromatography

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Area of Science:

  • Analytical Chemistry
  • Biopharmaceutical Analysis
  • Chromatography Method Development

Background:

  • Developing analytical assays in the biopharmaceutical industry is often time-consuming and relies on manual trial-and-error.
  • Existing methods lack efficiency, leading to extended development timelines and increased costs.

Purpose of the Study:

  • To present an automated analytical workflow for streamlined method development and optimization.
  • To demonstrate the application of an AI-based algorithm in chromatographic method development.
  • To reduce manual user intervention and analyst time in assay development.

Main Methods:

  • Implemented a feedback-controlled modeling approach for automated chromatographic method development.
  • Utilized readily available Liquid Chromatography (LC) instrumentation and software.
  • Focused on automatic optimization of mobile phase conditions and system control.

Main Results:

  • Achieved streamlined development and optimization of chromatographic methods from start to finish.
  • Significantly minimized the time requirement for analysts in method development.
  • Demonstrated successful application to challenging multicomponent mixtures including small molecules, peptides, proteins, and vaccine products.

Conclusions:

  • AI-based software and modern chromatography instrumentation accelerate the development of new separation assays.
  • The automated workflow leads to substantial cost savings, improved method robustness, and faster analytical turnaround.
  • This approach is applicable across various biopharmaceutical modalities.