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Efficient Sequential Detection of Two Antibiotics Using a Fiber-Optic Surface Plasmon Resonance Sensor.
Ze Zhao1, Huiting Yin2, Jingzhe Xiao1
1State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Sensors (Basel, Switzerland)
|April 13, 2024
Summary
This study presents a new sensor for detecting antibiotic residues enrofloxacin (Enro) and ciprofloxacin (Cip) in milk. The developed surface plasmon resonance (SPR) sensor offers high sensitivity and accurate detection, crucial for public safety.
Area of Science:
- Analytical Chemistry
- Biosensing Technology
- Food Safety
Background:
- Antibiotic residues in food pose a significant global public health concern.
- Simultaneous detection of multiple antibiotics is essential for effective monitoring.
- Existing methods may lack efficiency or specificity for combined antibiotic detection.
Purpose of the Study:
- To develop and validate an efficient surface plasmon resonance (SPR) sensor for the simultaneous detection of enrofloxacin (Enro) and ciprofloxacin (Cip) in milk.
- To establish a reliable method for quantifying these two common veterinary antibiotics.
Main Methods:
- Immobilization of enrofloxacin and ciprofloxacin antigens onto an optical fiber surface using dopamine polymerization and bovine serum albumin conjugates.
- Utilizing an immunosuppression principle for antibody-antigen binding.
- Development of standard curves for each antibiotic.
- Testing sensor performance including sensitivity, detection limits, and recovery rates in milk samples.
Main Results:
- The developed SPR sensor demonstrated high sensitivity (2900 nm/RIU).
- Achieved low detection limits: 1.20 ng/mL for enrofloxacin and 0.81 ng/mL for ciprofloxacin.
- Exhibited excellent recovery rates in milk samples (96.46-120.46% for Enro, 96.74-126.9% for Cip).
- Identified NaOH and Gly-HCl as optimal regeneration solutions for sensor reusability.
Conclusions:
- The proposed SPR sensor provides an efficient and sensitive platform for the simultaneous detection of enrofloxacin and ciprofloxacin in milk.
- This method contributes to ensuring food safety by enabling accurate monitoring of antibiotic residues.
- The sensor's regenerability suggests potential for routine application in quality control.
