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Interaction between intestinal and peripheral blood mononuclear cells.
Acta Chirurgica Scandinavica. Supplementum
|January 1, 1985
Summary
Crohn's disease alters intestinal immune regulation, with inflamed tissues showing increased immune cells. T cells from normal gut enhance IgA production, while those from Crohn's disease do not, suggesting a local immune dysfunction.
Area of Science:
- Immunology
- Gastroenterology
- Cell Biology
Background:
- Effective methods exist for isolating immunocytes from human intestinal mucosa.
- Understanding immune cell populations in Crohn's disease (CD) is crucial for elucidating disease mechanisms.
Purpose of the Study:
- To classify immunocytes from Crohn's disease and control intestinal mucosa based on ultrastructure and cell-surface markers.
- To analyze the functional properties of mucosa-associated T cells in relation to immunoglobulin (Ig) production.
Main Methods:
- Isolation and classification of mononuclear cells from human intestinal mucosa using monoclonal antibodies.
- Analysis of T cell phenotypes (T4, T8) and HLA-DR expression.
- Functional assessment of T cells in a pokeweed-stimulated autologous B cell assay.
Main Results:
- No significant differences in immunocyte populations between control and unaffected Crohn's disease mucosa.
- Inflamed Crohn's disease mucosa showed increased surface-Ig+ cells, monocytes, and granulocytes.
- Mucosa-associated T cells from normal individuals enhanced IgA production, while those from Crohn's disease patients did not.
Conclusions:
- Crohn's disease is associated with altered isotypic immunoregulation in the intestinal mucosa.
- A shift in the local immune response from IgA to IgG and IgM may contribute to antibody-dependent cytotoxicity against enterocytes.
- Peripheral T cells from Crohn's disease patients favor IgA isotype expression, contrasting with local findings.