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Mutagenic cholesterol preparations.

L L Smith, V B Smart, G A Ansari

    Mutation Research
    |September 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Commercial cholesterol samples contain mutagenic compounds linked to oxidation. Pure cholesterol becomes mutagenic after heating or gamma radiation exposure, indicating potential health risks from cholesterol degradation products.

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    Area of Science:

    • Biochemistry
    • Toxicology
    • Food Science

    Background:

    • Commercial cholesterol samples, both USP and reagent-grade, exhibit mutagenic properties.
    • These mutagenic properties are associated with polar cholesterol autoxidation products.
    • The specific identity of these mutagenic components remains unelucidated.

    Purpose of the Study:

    • To investigate the mutagenicity of naturally air-aged commercial cholesterol.
    • To explore the factors contributing to cholesterol-induced mutagenicity.
    • To understand the potential health implications of cholesterol degradation products.

    Main Methods:

    • Bacterial mutagenicity assays using Salmonella typhimurium strains (TA1537, TA1538, TA98).
    • Analysis of polar cholesterol autoxidation products.

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  • Exposure of pure cholesterol to heat (70°C in air) and gamma radiation (60Co).

    • Main Results:

    • Air-aged cholesterol samples demonstrated mutagenicity towards specific Salmonella strains.
    • Pure cholesterol, initially nonmutagenic, became mutagenic after thermal or radiation treatment.
    • Mutagenicity was linked to polar cholesterol autoxidation products.

    Conclusions:

    • Cholesterol autoxidation generates mutagenic compounds.
    • Both natural aging and artificial treatments (heat, radiation) can induce mutagenicity in cholesterol.
    • Further research is needed to identify the specific mutagenic agents and assess their toxicological significance.