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Related Experiment Video

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Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
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Determination of Multiclass Antibiotics in Fish Muscle Using a QuEChERS-UHPLC-MS/MS Method.

Yousra Aissaoui1,2, Gabriel Jiménez-Skrzypek2,3, Javier González-Sálamo2,3

  • 1Useful Materials Laboratory (LMU), National Institute for Physical and Chemical Research and Analysis (INRAP), Ariana 2020, Tunisia.

Foods (Basel, Switzerland)
|April 13, 2024
PubMed
Summary

Antibiotic residues in farmed fish are a concern. A new method detected minimal levels of oxolinic acid and tilmicosin in European sea bass, below safety limits, indicating safe aquaculture practices.

Keywords:
QuEChERSantibioticsfarmed fishmass spectrometryquinolones

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Area of Science:

  • Food safety analysis
  • Aquaculture science
  • Analytical chemistry

Background:

  • Global fish demand drives aquaculture, necessitating antibiotic use for disease prevention.
  • Antibiotic residues in farmed fish pose potential human health risks.
  • Monitoring antibiotic residues is crucial for ensuring food safety.

Purpose of the Study:

  • To develop and validate a sensitive method for detecting multiple antibiotic classes in farmed fish.
  • To assess the levels of specific antibiotics in European sea bass and gilt-head sea bream.
  • To ensure compliance with European Union (EU) maximum residue limits.

Main Methods:

  • Utilized the QuEChERS (Quick, Easy, Cheap, Rugged, and Safe) extraction technique.
  • Employed ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) for precise quantification.
  • Analyzed muscle tissue from European sea bass and gilt-head sea bream.

Main Results:

  • The validated method showed good recovery rates (70-120%) and met EU quantification limits.
  • Analysis of twenty fish samples revealed most antibiotics were below detection limits.
  • Trace amounts of oxolinic acid and tilmicosin were found in European sea bass, but below the method's limit of quantification.

Conclusions:

  • The developed QuEChERS-UHPLC-MS/MS method is effective for antibiotic residue analysis in farmed fish.
  • Detected antibiotic levels in the studied fish samples were below established safety thresholds.
  • Findings suggest current aquaculture practices, regarding the monitored antibiotics, pose minimal risk to human health.