Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ligand Binding and Linkage

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Antibiotic Resistance: From the Bench to Patients, 2.0.

Antibiotics (Basel, Switzerland)·2026

Same author

Native MS and ligand observed NMR uncovers subtle SLiM binding variations that mediate HSP90-Hop PPI modulation.

RSC chemical biology·2026

Same author

Multifactorial Perspective for Greening Solid-Phase Peptide Synthesis: Rigid Polyacrylate Macroporous Resin in Combination with Green Solvents.

ChemSusChem·2026

Same author

Short Antimicrobial Peptides Based on Arginine and Tryptophan: Agents with Potential in Combating Resistant Pathogens.

ACS omega·2026

Same author

2025 FDA TIDES (Peptides and Oligonucleotides) Harvest.

Pharmaceuticals (Basel, Switzerland)·2026

Same author

Cyclover-Assisted Liquid-Phase Peptide Synthesis Using T3P® as a Green Coupling Reagent.

Organic letters·2026

Same journal

RETRACTED: Atta et al. Effect of Montmorillonite Nanogel Composite Fillers on the Protection Performance of Epoxy Coatings on Steel Pipelines. <i>Molecules</i> 2017, <i>22</i>, 905.

Molecules (Basel, Switzerland)·2026

Same journal

Correction: Chen et al. Chemical Composition of <i>Litsea pungens</i> Essential Oil and Its Potential Antioxidant and Antimicrobial Activities. <i>Molecules</i> 2023, <i>28</i>, 6835.

Molecules (Basel, Switzerland)·2026

Same journal

Correction: Ruan et al. Comparison of Extraction, Isolation, Purification, Structural Characterization and Immunomodulatory Activity of Polysaccharides from Two Species of <i>Cistanche</i>. <i>Molecules</i> 2025, <i>30</i>, 4754.

Molecules (Basel, Switzerland)·2026

Same journal

Correction: Li et al. Gastrodin Ameliorates Cognitive Dysfunction in Vascular Dementia Rats by Suppressing Ferroptosis via the Regulation of the Nrf2/Keap1-GPx4 Signaling Pathway. <i>Molecules</i> 2022, <i>27</i>, 6311.

Molecules (Basel, Switzerland)·2026

Same journal

Correction: Zueva et al. Steady-State Kinetics of Enzyme-Catalyzed Hydrolysis of Echothiophate, a P-S Bonded Organophosphorus as Monitored by Spectrofluorimetry. <i>Molecules</i> 2020, <i>25</i>, 1371.

Molecules (Basel, Switzerland)·2026

Same journal

1,4-Diazatriphenylene and Its Hetero-Fused Analogs: Synthesis and Applications.

Molecules (Basel, Switzerland)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 28, 2025

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Safety-Catch Linkers for Solid-Phase Peptide Synthesis.

Sikabwe Noki^1,2, Beatriz G de la Torre², Fernando Albericio^1,3

¹Peptide Science Laboratory, School of Chemistry and Physics, University of KwaZulu-Natal, Westville, Durban 4000, South Africa.

Molecules (Basel, Switzerland)

|April 13, 2024

Summary

This summary is machine-generated.

Safety-catch linkers enhance solid-phase peptide synthesis (SPPS) by enabling simultaneous use of tert-butoxycarbonyl (Boc) and fluorenylmethoxycarbonyl (Fmoc) protecting groups. These linkers offer versatile cleavage options, improving peptide synthesis efficiency.

Keywords:

SPPS oxidative SCLs protecting groups reductive acidolysis SCLs safety-catch linkers (SCLs)

More Related Videos

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

Related Experiment Videos

Last Updated: Jun 28, 2025

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

Area of Science:

Organic Chemistry
Biochemistry
Synthetic Chemistry

Background:

Solid-phase peptide synthesis (SPPS) is a cornerstone for producing peptides for research and industrial applications.
Continuous innovation in SPPS, including novel resins and reagents, has significantly improved its efficiency and scope.
Safety-catch linkers represent a key advancement, offering enhanced control over peptide cleavage.

Purpose of the Study:

To review the development and significance of safety-catch linkers in solid-phase peptide synthesis.
To highlight how safety-catch linkers facilitate the use of orthogonal protecting group strategies.
To discuss the impact of safety-catch linkers on peptide cleavage conditions.

Main Methods:

Review of literature on safety-catch linker chemistry and applications in SPPS.
Analysis of linker stability under various deprotection conditions.
Comparison of cleavage strategies enabled by safety-catch linkers.

Main Results:

Safety-catch linkers are stable during standard SPPS deprotection steps.
Alkylation of safety-catch linkers renders them labile to specific cleavage conditions (acidic or basic).
This lability allows for simultaneous application of Boc and Fmoc protecting group strategies.

Conclusions:

Safety-catch linkers provide a versatile tool for modern peptide synthesis.
They enable orthogonal cleavage strategies, accommodating both Boc and Fmoc chemistries.
These linkers significantly advance the capabilities and flexibility of SPPS.