New Thiosemicarbazide Derivatives with Multidirectional Biological Action

Area of Science:

• Medicinal Chemistry
• Parasitology
• Microbiology

Background:

• Microbial and parasitic infections remain a global health threat due to evolving resistance mechanisms.
• Continuous research is vital for developing novel pharmacotherapies against resistant pathogens.

Purpose of the Study:

• To synthesize new chemical entities derived from 3-trifluoromethylbenzoic acid hydrazide.
• To evaluate the biological activities, including anthelmintic, antibacterial, and antioxidant properties, of these novel compounds.

Main Methods:

• Synthesis of seven new compounds (six linear, one 1,2,4-triazole derivative).
• In silico prediction of biological activity using PASS software.
• In vitro testing for nematocidal activity against *Rhabditis* sp.
• Antibacterial assays using standard strains.
• Cytotoxicity assessment on two cell lines.
• Antioxidant activity evaluation.

Main Results:

• Successful synthesis of novel 3-trifluoromethylbenzoic acid hydrazide derivatives.
• Compounds exhibited promising anthelmintic and antibacterial potential.
• Two derivatives, featuring a 3-chlorophenyl substituent (linear and cyclic), displayed the highest efficacy.
• These compounds outperformed existing therapeutic drugs in preliminary tests.

Conclusions:

• The synthesized compounds possess significant anthelmintic and antibacterial properties.
• The 3-chlorophenyl substituted derivatives represent promising candidates for new drug development.
• Further investigation is warranted to explore their therapeutic potential against parasitic and bacterial infections.