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Updated: Jun 28, 2025

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

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Rel Family Transcription Factor NFAT5 Upregulates COX2 via HIF-1α Activity in Ishikawa and HEC1a Cells

Toshiyuki Okumura1,2, Janet P Raja Xavier1, Jana Pasternak1

  • 1Department of Women's Health, Tübingen University Hospital, D-72076 Tübingen, Germany.

International Journal of Molecular Sciences
|April 13, 2024
PubMed

Insights

Nuclear factor of activated T cells 5 (NFAT5) promotes endometrial cancer progression by activating the HIF-1α-COX2 pathway. This pathway

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Signaling

Background:

  • Nuclear factor of activated T cells 5 (NFAT5) and cyclooxygenase 2 (COX2) are implicated in various pathologies, including cancer.
  • The specific role of the NFAT5-COX2 pathway in human endometrial cancer remains unclear.

Purpose of the Study:

  • To investigate NFAT5 expression in endometrial tumors.
  • To determine if NFAT5 contributes to endometrial cancer progression.
  • To elucidate the molecular mechanisms underlying NFAT5's role.

Main Methods:

  • Immunohistochemistry to assess NFAT5 protein levels in endometrial cancer tissues.
  • Transfection of endometrial cancer cells (Ishikawa, HEC1a) with NFAT5 or empty plasmid.
  • RNA sequencing to identify genes regulated by NFAT5.
  • Reporter assays to evaluate HIF-1α promoter activity.

Main Results:

  • NFAT5 expression is significantly higher in high-grade versus low-grade endometrial cancer.
  • NFAT5 overexpression alters the expression of key genes, including cyclooxygenase 2 (COX2) and hypoxia-inducible factor 1α (HIF1A).
  • NFAT5 activation stimulates HIF-1α promoter activity, COX2 expression, and prostaglandin E2 (PGE2) levels.

Conclusions:

  • The NFAT5-HIF-1α-COX2 signaling axis is activated in endometrial cancer.
  • This axis plays a crucial role in promoting endometrial cancer progression.
Keywords:
cyclooxygenase 2endometrial cancerhypoxia inducible factor 1α

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