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Related Concept Videos

Vascular Spasm01:16

Vascular Spasm

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The vascular phase, also known as vasospasm, is the initial stage of hemostasis, crucial for preventing excessive bleeding when a blood vessel is injured. After a vessel is cut, nerves in the damaged area trigger pain and other sensory impulses. Simultaneously, the smooth muscles in the vessel wall contract, resulting in a vascular spasm. This contraction reduces the vessel's diameter at the injury site, slowing or stopping blood loss through the vessel wall. Vascular spasms typically last...
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Phases of Wound Repair01:28

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Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
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Updated: Jun 28, 2025

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
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Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

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Complement MASP-1 Modifies Endothelial Wound Healing.

Zsuzsanna Németh1, Flóra Demeter1, József Dobó2

  • 1Department of Internal Medicine and Haematology, Semmelweis University, 1085 Budapest, Hungary.

International Journal of Molecular Sciences
|April 13, 2024
PubMed
Summary
This summary is machine-generated.

Mannan-binding lectin-associated serine protease-1 (MASP-1) influences endothelial wound healing by altering gene expression and promoting angiogenesis. MASP-1 enhances cellular responses to mechanical wounding, impacting circulatory system repair.

Keywords:
CREB phosphorylationCa2+ responseMASP-1Matrigel®adhesion moleculesangiogenesisendothelial cellwound healing

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Area of Science:

  • Vascular Biology
  • Immunology
  • Cellular Biology

Background:

  • Endothelial wound healing is crucial for circulatory system maintenance.
  • The complement protein mannan-binding lectin-associated serine protease-1 (MASP-1) can activate endothelial cells.
  • MASP-1's role in conjunction with mechanical injury is not fully understood.

Purpose of the Study:

  • To investigate the combined effects of mechanical wounding and MASP-1 on endothelial cells.
  • To elucidate MASP-1's impact on wound-healing and angiogenesis-related gene expression.
  • To understand MASP-1's role in endothelial cell responses to injury.

Main Methods:

  • Transcriptomic analysis to assess gene expression changes.
  • Calcium (Ca2+) mobilization assays.
  • Western blotting for CREB phosphorylation.
  • Immunofluorescence for ICAM-1 and VCAM-1 expression.
  • Matrigel® assay to evaluate angiogenesis.

Main Results:

  • MASP-1 alters expression of wound-healing and angiogenesis genes.
  • Mechanical wounding inhibits MASP-1-induced Ca2+ mobilization but enhances CREB phosphorylation.
  • MASP-1 increases VCAM-1 expression and facilitates capillary network breakdown in angiogenesis.
  • MASP-1 plays a role in later stages of angiogenesis.

Conclusions:

  • MASP-1 significantly influences endothelial wound healing and angiogenesis.
  • Combined mechanical wounding and MASP-1 activation modulate cellular signaling pathways.
  • Complement MASP-1 activation is important in vascular repair processes.