Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.1K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.1K
Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-regional transcriptomic profiling reveals divergent molecular mechanisms in ALS-related neurodegeneration.

PLoS genetics·2026
Same author

Non-canonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy.

eLife·2026
Same author

Ubiquitin ligase ITCH regulates life cycle of SARS-CoV-2 virus.

eLife·2026
Same author

Corrigendum to CRISPR/Cas13d targeting suppresses repeat-associated non-AUG translation of C9orf72 hexanucleotide repeat RNA.

The Journal of clinical investigation·2026
Same author

UBQLN2 links proteotoxicity with lipid metabolism in neurodegeneration.

Nature neuroscience·2026
Same author

A Deep Quantitative Proteome Turnover Platform for Human iPSC-derived Neurons.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 28, 2025

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

8.7K

Protocol to identify DNA-binding proteins recognizing nucleotide repeat dsDNAs.

Zhiyuan Huang1, Yixin Zhou1, Yang Liu1

  • 1Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.

STAR Protocols
|April 13, 2024
PubMed
Summary

This study introduces a method to identify DNA-binding proteins that interact with specific DNA repeats. This helps understand the genetic causes of C9orf72 amyotrophic lateral sclerosis and frontotemporal dementia.

Keywords:
Cell BiologyCell cultureCell separation/fractionationCell-based AssaysMolecular BiologyMolecular/Chemical ProbesProtein BiochemistryProtein expression and purification

More Related Videos

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

966
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

6.7K

Related Experiment Videos

Last Updated: Jun 28, 2025

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

8.7K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

966
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

6.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Neuroscience

Background:

  • DNA-binding proteins are crucial for cellular functions like growth regulation and chromatin organization.
  • Expansion of hexanucleotide repeats is the primary genetic cause of C9orf72-linked amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).

Purpose of the Study:

  • To develop and present a protocol for identifying proteins that specifically bind to hexanucleotide repeat DNA sequences.
  • To investigate the molecular mechanisms underlying C9orf72-ALS and FTD by characterizing interacting DNA-binding proteins.

Main Methods:

  • Utilized a SILAC (stable isotope labelling by amino acids in cell culture)-based approach to isolate and identify DNA-binding proteins.
  • Employed electrophoretic mobility shift assay (EMSA) for validation of protein-DNA interactions.

Main Results:

  • Successfully identified proteins that specifically recognize double-stranded hexanucleotide repeat DNAs.
  • The protocol provides a robust method for discovering novel DNA-binding proteins involved in repeat expansion disorders.

Conclusions:

  • The presented protocol enables the discovery of DNA-binding proteins interacting with disease-associated repeat expansions.
  • This research contributes to understanding the pathogenesis of C9orf72-linked neurodegenerative diseases.