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Updated: Jun 28, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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ZNF146 regulates cell cycle progression via TFDP1 and DEPDC1B in ovarian cancer cells

Ruixue Zhao1, Nana Song2, Xin Ning1

  • 1Department of Gynaecology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, P.R. China.

Reproduction (Cambridge, England)
|April 13, 2024
PubMed

Insights

This study reveals a new pathway involving Zinc finger protein 146 (ZNF146), Transcription factor Dp-1 (TFDP1), and DEP domain-containing protein 1B (DEPDC1B) that regulates the cell cycle in ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Ovarian cancer (OC) is a lethal gynecological malignancy.
  • Aberrant cell cycle progression is a hallmark of tumorigenesis.
  • The role of Transcription factor Dp-1 (TFDP1) in OC requires further elucidation.

Purpose of the Study:

  • To investigate the regulatory role and mechanism of TFDP1 in ovarian cancer.
  • To identify novel regulators of the cell cycle in OC.

Main Methods:

  • Analysis of TFDP1 expression in OC tissues and cells.
  • Gene silencing experiments (ZNF146 knockdown, TFDP1 silencing).
  • Assessment of cell cycle progression and tumor growth in vivo.

Main Results:

  • TFDP1 expression is elevated in OC and promotes cell proliferation and cell cycle entry.
  • ZNF146 knockdown inhibits OC cell growth by suppressing TFDP1 transcription.
  • TFDP1 activates DEP domain-containing protein 1B (DEPDC1B) expression, promoting G2/M phase transition and tumor progression.

Conclusions:

  • The ZNF146/TFDP1/DEPDC1B axis is a critical regulator of the cell cycle in ovarian cancer.
  • Targeting this axis may offer novel therapeutic strategies for OC.

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