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Efficacy of Native Bacillus thuringiensis against Mosquito Vector
Ganga Gc1, Kshama Parajuli2, Ishan Gautam3
1Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
Journal of Nepal Health Research Council
|April 14, 2024
Summary
Researchers isolated a native Bacillus thuringiensis strain from Nepal
Area of Science:
- Microbiology
- Environmental Science
- Medical Entomology
Background:
- Larval source management is key for controlling mosquito-borne diseases.
- Bacillus thuringiensis (Bt) produces insecticidal proteins effective against mosquito larvae.
- Nepal lacks readily available larvicidal Bt for disease control.
Purpose of the Study:
- To isolate and identify larvicidal Bacillus thuringiensis from Nepalese soil.
- To assess the efficacy of native Bt strains against mosquito larvae.
- To develop a sustainable method for mosquito control in Nepal.
Main Methods:
- Soil samples were collected and screened for Bacillus thuringiensis using acetate selection.
- Crystal proteins were identified microscopically.
- Bioassays were conducted on mosquito larvae using isolated Bt strains.
Main Results:
- Over 1300 Bt strains were isolated, with 55% producing spherical crystals.
- A single native strain, 14P2A, demonstrated 100% larval mortality.
- Lethal concentration (LC50) was determined at 32.35 ppm and LC90 at 46.77 ppm.
Conclusions:
- Native Bacillus thuringiensis strains can effectively kill mosquito larvae.
- The isolated strain 14P2A shows potential for mosquito-borne disease control in Nepal.
- Integrating native Bt into larval source management programs is recommended.
