Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

2.8K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
2.8K
COPD: Pathogenesis and Clinical Features01:20

COPD: Pathogenesis and Clinical Features

267
Chronic obstructive pulmonary disease (COPD) is a group of lung conditions that progressively worsen over time, including chronic bronchitis and emphysema. This cluster of diseases collectively leads to a gradual and irreversible decline in lung function over time.
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...
267
Chronic Obstructive Pulmonary Disease-I: Introduction01:20

Chronic Obstructive Pulmonary Disease-I: Introduction

2.8K
Chronic Obstructive Pulmonary Disease (COPD) is a long-lasting respiratory condition requiring continuous attention and care. It is a progressive lung disease that leads to breathing challenges due to airflow obstruction. It manifests as persistent respiratory symptoms and restricted airflow resulting from abnormalities in the airways and alveoli, usually due to long-term exposure to harmful particles or gases. COPD mainly consists of two primary conditions: emphysema and chronic bronchitis.
2.8K
Chronic Obstructive Pulmonary Disease-III: Symptoms and Complications.01:25

Chronic Obstructive Pulmonary Disease-III: Symptoms and Complications.

2.7K
Understanding the variety of primary symptoms and systemic complications that characterize chronic obstructive pulmonary disease (COPD) is crucial for healthcare professionals.
Symptoms of COPD can be classified as primary or systemic. Primary symptoms relate to reduced airflow, while systemic or extrapulmonary symptoms relate to COPD's broader impact on the body.
Primary Symptoms of COPD:
2.7K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Chronic Obstructive Pulmonary Disease01:22

Chronic Obstructive Pulmonary Disease

1.2K
COPD is defined as a heterogeneous lung condition marked by persistent respiratory symptoms such as dyspnea, cough, and sputum production, caused by abnormalities in the airways that cause airflow obstruction.
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
1.2K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Hhip Protein Interactions In Lung Cells Provide Insight Into Copd Pathogenesis.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Hhip Protein Interactions In Lung Cells Provide Insight Into Copd Pathogenesis.

Related Experiment Video

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

18.1K

HHIP protein interactions in lung cells provide insight into COPD pathogenesis.

Dávid Deritei, Hiroyuki Inuzuka, Peter J Castaldi

    Biorxiv : the Preprint Server for Biology
    |April 15, 2024

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    Researchers mapped protein interactions for HHIP, a gene linked to Chronic Obstructive Pulmonary Disease (COPD). This reveals new connections between COPD genes and implicates HHIP in oxidative stress responses.

    More Related Videos

    Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections
    07:17

    Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

    Published on: October 6, 2023

    5.5K
    Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
    10:21

    Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

    Published on: September 20, 2024

    424

    Related Experiment Videos

    Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
    09:17

    Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

    Published on: August 2, 2018

    18.1K
    Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections
    07:17

    Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

    Published on: October 6, 2023

    5.5K
    Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
    10:21

    Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

    Published on: September 20, 2024

    424

    Area of Science:

    • Genomics and Bioinformatics
    • Molecular Biology
    • Pulmonary Medicine

    Background:

    • Chronic Obstructive Pulmonary Disease (COPD) is a major global health concern with both environmental and genetic risk factors.
    • Genome-Wide Association Studies (GWASes) have identified over 80 genetic loci associated with COPD, but the biological networks connecting these genes remain poorly understood.
    • Understanding gene networks is crucial for elucidating complex disease pathogenesis.

    Approach:

    • Affinity purification mass spectrometry (AP-MS) was employed to identify protein interactions of HHIP, a known COPD GWAS gene within the sonic hedgehog pathway.
    • A protein-protein interaction network, HUBRIS, was constructed using 8 public databases to analyze HHIP's network neighborhood and proximity to other COPD GWAS genes.
    • Interactions were investigated in two relevant lung cell lines (IMR90 and 16HBE) to identify common and cell-type-specific interactors.

    Key Points:

    • Newly identified protein interactions for HHIP were discovered, including cell type-specific ones.
    • These interactions shorten the network distance between HHIP and other COPD GWAS genes like DSP, MFAP2, TET2, and FBLN5.
    • Validated interactions with CAVIN1 (in IMR90 cells) and TP53 (in 16HBE cells) were found, suggesting roles in extracellular matrix organization and oxidative stress response.

    Conclusions:

    • The study provides novel insights into the functional role of HHIP in COPD pathogenesis by uncovering its protein interaction network.
    • The findings highlight the involvement of HHIP in pathways related to extracellular matrix organization and response to oxidative stress.
    • This research contributes to a better understanding of the complex genetic architecture of COPD and identifies potential therapeutic targets.