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Updated: Jul 13, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Temporal stability of tongue microbiota in older patients - A pilot study
Fa-Tzu Tsai1, Cheng-Chieh Yang1,2, Yu-Cheng Lin1,3
1College of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Background/Purpose:
Healthy states of human microbiota depend on a stable community of symbiotic microbes irrespective of external challenges from the environment. Thus, long-term stability of the oral microbiota is of importance, particularly for older patient populations.
Materials And Methods:
We used next-generation sequencing (NGS) to examine the tongue microbiota of 18 individuals receiving long-term care over a 10-month period.
Results:
Beta diversity analysis demonstrated temporal stability of the tongue microbiota, as microbial compositions from all time points were indistinguishable from each other (P = 0.0887). However, significant individual variation in microbial composition (P = 0.0001) was observed, underscoring the presence of a unique microbial profile for each patient.
Conclusion:
The temporal dynamics of tongue microbiota exhibit long-term stability, providing diagnostic implications for oral diseases within older patient populations.
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