Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells.

R Derynck, J A Jarrett, E Y Chen

    Nature
    |August 22, 1985
    PubMed
    Summary

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cardioprotective actions of transforming growth factor-β.

    Trends in cardiovascular medicine·2011
    Same author

    Functional redundancy of TGF-beta family type I receptors and receptor-Smads in mediating anti-Mullerian hormone-induced Mullerian duct regression in the mouse.

    Biology of reproduction·2008
    Same author

    Smad3 deficiency inhibits v-ras-induced transformation by suppression of JNK MAPK signaling and increased farnesyl transferase inhibition.

    Oncogene·2007
    Same author

    Targeted mutagenesis of Smad1 reveals an essential role in chorioallantoic fusion.

    Developmental biology·2002
    Same author

    Meeting report: signaling schemes for TGF-beta.

    Science's STKE : signal transduction knowledge environment·2001
    Same author

    Suppressor and oncogenic roles of transforming growth factor-beta and its signaling pathways in tumorigenesis.

    Advances in cancer research·2001

    Researchers identified complementary DNA (cDNA) clones for human transforming growth factor-beta (TGF-beta) using its amino-acid sequence. This revealed TGF-beta is synthesized from a longer precursor polypeptide, with messenger RNA found in diverse cell types.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Transforming Growth Factor-beta (TGF-beta) is a crucial signaling molecule.
    • Understanding the genetic basis of TGF-beta is essential for cell growth and differentiation research.

    Purpose of the Study:

    • To identify cDNA clones encoding human TGF-beta.
    • To elucidate the precursor structure and synthesis of TGF-beta.

    Main Methods:

    • Partial amino-acid sequencing of purified human TGF-beta.
    • cDNA library screening and sequencing.

    Main Results:

    • A series of cDNA clones encoding TGF-beta were identified.
    • The 112-amino acid monomeric TGF-beta is derived from a larger precursor polypeptide.

    Related Experiment Videos

  • TGF-beta messenger RNA is present in various normal and transformed cells.

    • Conclusions:

    • The identified cDNA sequence provides insights into TGF-beta biosynthesis.
    • TGF-beta synthesis involves proteolytic processing from a precursor.
    • TGF-beta expression is widespread across different cell types.