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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 13, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

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High-Density Microporous Drainage-Integrating Sheath Flow Generator for Streamlining Microfluidic Cell Sorting

Ayumi Hayashi¹, Runa Hemmi¹, Yuhei Saito¹

¹Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.

Analytical Chemistry

|April 15, 2024

Summary

A novel sheath flow generator using a polydimethylsiloxane (PDMS) sponge simplifies microfluidic cell sorting. This innovation enhances system usability and streamlines cell and particle separation processes.

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Area of Science:

Biotechnology
Microfluidics
Materials Science

Background:

Microfluidic cell sorting systems face challenges with complexity and operability.
Existing systems require intricate operational procedures, limiting widespread adoption.

Purpose of the Study:

To develop a simplified sheath flow generator for microfluidic cell sorters.
To enhance the usability and efficiency of microfluidic cell sorting platforms.

Main Methods:

Fabrication of a sponge-embedded substrate using PMMA microparticles as sacrificial porogens.
Integration of a polydimethylsiloxane (PDMS) sponge with interconnected micropores into microchannels.
Investigation of microchannel geometry and flow velocity effects on sheath flow generation.

Main Results:

Successful generation of sheath flow using drained fluid from the integrated PDMS sponge.
Demonstrated effective cell and particle sorting using model particles, blood cells, and tumor cells.
Validated the influence of microchannel design and flow rates on sheath flow performance.

Conclusions:

The developed sheath flow generator significantly simplifies microfluidic cell sorting operations.
This technique improves the versatility and operability of microfluidic cell sorting systems.
The approach offers a streamlined solution for advanced cell and particle separation technologies.