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Coronavirus

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...

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Related Experiment Video

Updated: Jul 3, 2026

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

Sensitive methods for detection of SARS-CoV-2 RNA.

Xi Chen¹, Simin Xia¹

¹The HIT Center for Life Sciences (HCLS), Harbin Institute of Technology, Harbin, Heilongjiang Province, People's Republic of China.

Methods in Microbiology

|April 15, 2024

Summary

This summary is machine-generated.

Sensitive detection of SARS-CoV-2 RNA is crucial for pandemic control. The reverse transcription recombinase polymerase amplification (RT-RPA) method offers high sensitivity for rapid, cost-effective COVID-19 diagnostics, even with pooled samples.

Keywords:

Exo probe Lateral flow Nfo probe RT-RPA SARS-CoV-2 RNA Single-copy sensitivity

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Area of Science:

Molecular Biology
Virology
Biotechnology

Background:

The COVID-19 pandemic necessitates sensitive and rapid SARS-CoV-2 detection for public health.
Community monitoring often involves sample pooling, requiring highly sensitive diagnostic methods to overcome RNA dilution.
Isothermal nucleic acid amplification offers a faster, more accessible alternative to traditional PCR.

Purpose of the Study:

To explore the application of reverse transcription recombinase polymerase amplification (RT-RPA) for sensitive SARS-CoV-2 RNA detection.
To summarize and compare existing RT-RPA based SARS-CoV-2 detection methods.
To provide guidance on designing ultrasensitive RT-RPA assays and detail a modified RT-RPA (RT-ERA) method.

Main Methods:

Review and comparison of published RT-RPA based SARS-CoV-2 detection methods, focusing on parameters, primers, and probes.
Discussion of key considerations for designing ultrasensitive RT-RPA assays.
Detailed description of a developed modified RT-RPA (RT-ERA) assay for single-copy sensitivity detection of SARS-CoV-2 RNA.

Main Results:

RT-RPA demonstrates outstanding sensitivity, achieving single-copy detection limits in test reactions.
Comparison of various RT-RPA methods highlights differences in detection parameters and primer/probe designs.
A modified RT-RPA (RT-ERA) method achieved single-copy sensitivity for SARS-CoV-2 RNA detection.

Conclusions:

RT-RPA is a highly promising isothermal amplification technique for sensitive and rapid SARS-CoV-2 detection.
The development of modified RT-RPA assays like RT-ERA can further enhance sensitivity for diagnostics and surveillance.
RT-RPA technology offers a viable, cost-effective solution for widespread SARS-CoV-2 screening and monitoring.