Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

GPCR Desensitization01:12

GPCR Desensitization

6.0K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
6.0K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Rapid Fabrication of Highly Customizable Test Chambers for Behavioral Experiments with Small Aquatic Model Organisms.

Zebrafish·2026
Same journal

Essential Oil of <i>Varronia curassavica</i> (Cordiaceae) as an Anesthetic and Sedative for Zebrafish.

Zebrafish·2026
Same journal

Dynamic Population Breeding: A Structured Colony Management Strategy to Improve Reproductive Performance and Early Survival in <i>Nothobranchius furzeri</i>.

Zebrafish·2026
Same journal

Auxin Inducible Protein Degradation in Zebrafish.

Zebrafish·2026
Same journal

<i>Corrigendum to:</i> A Zebrafish Heart Failure Model for Assessing Therapeutic Agents.

Zebrafish·2026
Same journal

Global Landscape of Zebrafish Research (2000-2025): A Bibliometric Analysis of Publication Trends, Collaboration, and Thematic Evolution.

Zebrafish·2026

Related Experiment Video

Updated: Jun 28, 2025

Inducible and Reversible Dominant-negative DN Protein Inhibition
08:35

Inducible and Reversible Dominant-negative DN Protein Inhibition

Published on: January 7, 2019

8.4K

Teaching Fin Regeneration Using a Dominant Negative Receptor.

Greta M Mahler1, Barbara E Sisson1

  • 1Department of Biology, Ripon College, Ripon, Wisconsin, USA.

Zebrafish
|April 15, 2024
PubMed
Summary

This study uses zebrafish to teach tissue regeneration, observing how activating a dominant-negative fibroblast growth factor receptor 1 (FGFR1) transgene affects fin regrowth. Students gain hands-on experience and critical thinking skills in regeneration research.

Keywords:
FGF signalingheat shocksignal transduction pathwaytransgenezebrafish

More Related Videos

Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform RpEGEN for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium
13:12

Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform RpEGEN for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

Published on: March 2, 2022

2.5K
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

8.7K

Related Experiment Videos

Last Updated: Jun 28, 2025

Inducible and Reversible Dominant-negative DN Protein Inhibition
08:35

Inducible and Reversible Dominant-negative DN Protein Inhibition

Published on: January 7, 2019

8.4K
Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform RpEGEN for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium
13:12

Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform RpEGEN for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

Published on: March 2, 2022

2.5K
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

8.7K

Area of Science:

  • Developmental Biology
  • Genetics
  • Educational Science

Background:

  • Zebrafish are increasingly used as model organisms in educational settings.
  • Understanding tissue regeneration and its genetic regulation is crucial in biology.
  • Previous research highlights the role of fibroblast growth factor receptors (FGFRs) in developmental processes.

Purpose of the Study:

  • To design and implement a laboratory experiment for students to investigate tissue regeneration in zebrafish.
  • To demonstrate the effects of manipulating fibroblast growth factor receptor 1 (FGFR1) signaling on caudal fin regeneration.
  • To enhance students' understanding of gene function and deductive reasoning through a hands-on and literature-based approach.

Main Methods:

  • Students performed caudal fin amputations on zebrafish.
  • A dominant-negative fgfr1 transgene was activated using heat shock to observe its effects.
  • Students conducted visual observations of fin regeneration over one week.
  • A literature-based component required students to research genes involved in regeneration inhibition.

Main Results:

  • Observed differences in caudal fin regeneration between zebrafish with activated and unactivated fgfr1 transgenes.
  • Students successfully applied deductive reasoning to infer gene functions related to regeneration.
  • The experiment provided a tangible model for understanding gene-environment interactions in development.

Conclusions:

  • The experiment effectively illustrates the role of FGFR1 signaling in zebrafish fin regeneration.
  • Combining hands-on activities with literature research enhances student engagement and learning in developmental biology.
  • This model organism-based experiment fosters critical thinking and practical skills in aspiring scientists.