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Atomic Absorption Spectroscopy: Interference

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...

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Related Experiment Video

Updated: Jun 28, 2025

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Published on: January 27, 2014

Tackling assay interference associated with small molecules.

Lu Tan¹, Steffen Hirte^2,3, Vincenzo Palmacci^2,3

¹Drug Discovery Sciences, Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria.

Nature Reviews. Chemistry

|April 15, 2024

Summary

This summary is machine-generated.

Assay interference from compound properties poses challenges in drug discovery. This review covers experimental and in silico methods to identify and mitigate these issues for reliable screening results.

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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Area of Science:

Biochemistry
Cell Biology
Drug Discovery

Background:

Biochemical and cell-based assays are crucial for developing new drugs, agrochemicals, and cosmetics.
Assay interference from compound properties (e.g., aggregation, reactivity) leads to false readouts, hindering screening of synthetic and natural products.

Purpose of the Study:

To review experimental and in silico methods for addressing assay interference.
To discuss strategies for integrating experimental and theoretical approaches.
To provide best practice recommendations for reliable compound screening.

Main Methods:

Overview of existing experimental techniques for assay interference detection.
Focus on theoretical (in silico) methods for predicting and managing interference.
Discussion of combined experimental and in silico strategies.

Main Results:

Experimental approaches offer powerful solutions but have limitations.
In silico methods are increasingly valuable for tackling assay interference.
Integrated strategies enhance the reliability of screening campaigns.

Conclusions:

Addressing assay interference is critical for accurate and efficient compound screening.
A combination of experimental and in silico methods provides a robust framework.
Future developments will likely enhance the predictive power of theoretical approaches.