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Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

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Leaching Approach for β-Glucosidase Extraction from Fermented Rice Husk in Solid State Cultivation by Aspergillus

P Suresh Yadav¹, B V Siva Prasad¹, M Subhosh Chandra²

¹Department of Microbiology, Yogi Vemana University, Kadapa, Andhra Pradesh, India.

Current Microbiology

|April 15, 2024

Summary

Agrowaste conversion into valuable products like beta-glucosidase is crucial. Researchers optimized enzyme extraction from rice husk using acetate buffer, achieving high yields for industrial applications.

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Area of Science:

Biotechnology
Environmental Science
Enzyme Engineering

Background:

Agrowaste disposal poses environmental challenges in developing nations.
Enzymes like beta-glucosidase are vital for converting waste into valuable products.
Beta-glucosidase has diverse applications in industries such as food, textiles, and biofuels.

Purpose of the Study:

To produce beta-glucosidase using Aspergillus protuberus on rice husk via solid-state fermentation.
To optimize the extraction solvent and conditions for maximum beta-glucosidase recovery.

Main Methods:

Solid-state fermentation (SSF) of rice husk by Aspergillus protuberus.
Screening various solvents for beta-glucosidase leaching efficacy.
Optimization of extraction parameters including buffer type, pH, volume, and washing cycles.

Main Results:

Acetate buffer (0.02 M, pH 5.0) was identified as the most effective solvent.
Optimized extraction involved two washes with acetate buffer (1 g rice husk: 5 ml buffer) for 30 min each.
Maximum beta-glucosidase recovery achieved was 41.95 U/g of rice husk.

Conclusions:

Effective beta-glucosidase production and extraction from rice husk are feasible using Aspergillus protuberus and optimized acetate buffer leaching.
This method offers a sustainable approach to valorize agrowaste and obtain industrially relevant enzymes.