Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Super-resolution mapping reveals NAD⁺-delivering probiotic extracellular vesicles as nanotherapeutics for organelle protection and inflammation control.

Journal of nanobiotechnology·2026
Same author

Structure of the Huntingtin F-actin complex reveals its role in cytoskeleton organization.

Science advances·2025
Same author

Engineered MSC-EVs loaded with BDNF-enhancing neuropeptides via a non-disruptive method enhance post-stroke neuroregeneration via intranasal delivery.

Journal of nanobiotechnology·2025
Same author

Inverted PAINT for Material-Specific Super-Resolution Fluorescence Imaging of Semiconductors.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Super-Resolution Imaging of Semiconductor Nanopatterns Using the Non-Bridging-Oxygen-Hole-Center-Based Photoluminescence Enhancement Effect.

Journal of the American Chemical Society·2025
Same author

Advancing biosensing through super-resolution fluorescence microscopy.

Biosensors & bioelectronics·2025

Related Experiment Video

Updated: Jun 28, 2025

Ground State Depletion Super-resolution Imaging in Mammalian Cells
07:55

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

7.2K

Photoswitching Reagent for Super-Resolution Fluorescence Microscopy.

Ga-Eun Go1, Uidon Jeong1, Hyunbum Park1

  • 1Department of Chemistry, Hanyang University, Seoul, 04763, Republic of Korea.

Angewandte Chemie (International Ed. in English)
|April 15, 2024
PubMed
Summary

Understanding single-molecule photoswitching mechanisms in super-resolution microscopy is key. This study reveals switch-off depends on reagent nucleophilicity and switch-on on laser power and bond energy.

Keywords:
Photoswitching mechanismPhotoswitching reagentSingle-molecule localization microscopySuper-resolution microscopy

More Related Videos

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
11:21

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Published on: June 26, 2010

12.2K
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
08:18

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

Published on: June 16, 2020

7.4K

Related Experiment Videos

Last Updated: Jun 28, 2025

Ground State Depletion Super-resolution Imaging in Mammalian Cells
07:55

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

7.2K
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
11:21

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Published on: June 26, 2010

12.2K
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
08:18

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

Published on: June 16, 2020

7.4K

Area of Science:

  • Optical Microscopy
  • Biophysics
  • Chemical Physics

Background:

  • Single-molecule localization microscopy (SMLM) offers super-resolution imaging beyond the diffraction limit.
  • Fluorophore photoswitching is essential for SMLM, enabling nanoscale visualization of cellular structures and dynamics.
  • Optimizing photoswitching is critical for accurate molecular position determination in SMLM.

Purpose of the Study:

  • To elucidate the general on and off photoswitching mechanisms of single dye molecules.
  • To evaluate various photoswitching reagents for their impact on SMLM performance.
  • To provide a foundation for designing enhanced photoswitching reagents for SMLM.

Main Methods:

  • Systematic measurement of single-molecule fluorescence on and off rates (k_on, k_off).
  • Evaluation of diverse photoswitching reagents.
  • Theoretical calculations of photoswitching reagent-fluorophore pair structures.

Main Results:

  • The switch-off mechanism is primarily governed by the photoswitching reagent's nucleophilicity.
  • The switch-on mechanism involves a two-photon-induced dissociation process.
  • Switch-on is influenced by illuminating laser power and the pair's bond dissociation energy.

Conclusions:

  • This research deepens the understanding of molecular photoswitching in SMLM.
  • Findings pave the way for developing improved photoswitching reagents.
  • Potential applications extend to materials science and chemistry.