Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

De Novo Biosynthesis of Valinomycin From Glucose Using In Vitro Reconstituted Hybrid Pathways.

Chembiochem : a European journal of chemical biology·2026

Same author

SARGE: Serine Integrase-Assisted Rapid Genome Integration through Cassette Exchange in <i>Escherichia coli</i>.

ACS synthetic biology·2026

Same author

A framework for building a synthetic cell from the SynCell Asia Initiative.

Nature biotechnology·2026

Same author

Engineering LmrR protein for L-proline-based asymmetric aldol biocatalysis.

Nature communications·2026

Same author

Reconstituting transcription-translation-coupled DNA replication within complex in vitro biological systems.

Nature communications·2025

Same author

Functional Characterization of a Diazo-Forming Enzyme in Meroterpenoid Biosynthesis.

Chembiochem : a European journal of chemical biology·2025

Search research articles

Related Experiment Video

Updated: Jun 28, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Rainbow screening: Chromoproteins enable visualized molecular cloning.

Fang Ba¹, Yufei Zhang¹, Wan-Qiu Liu¹

¹School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.

Biotechnology Journal

|April 16, 2024

Summary

Rainbow Screening simplifies molecular cloning by using chromoproteins to visually identify correct bacterial colonies without extra genetic steps. This method enhances cloning efficiency and accuracy for biotechnology applications.

Keywords:

DNA assembly chromoprotein molecular cloning plasmid construction synthetic biology

More Related Videos

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

Published on: September 20, 2013

Related Experiment Videos

Last Updated: Jun 28, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

Published on: September 20, 2013

Area of Science:

Biotechnology
Synthetic Biology
Molecular Biology

Background:

Molecular cloning is essential for assembling DNA fragments into plasmids for replication in host cells.
Current screening methods like blue-white screening require additional genetic manipulations and costs.
There is a need for simplified, cost-effective methods for visualizing successful molecular cloning.

Purpose of the Study:

To develop a simplified and cost-effective method for visualized molecular cloning.
To introduce Rainbow Screening, combining Gibson Assembly with chromoproteins for visual colony identification.
To eliminate the need for additional genetic manipulations and costs in molecular cloning screening.

Main Methods:

Developed Rainbow Screening, integrating Gibson Assembly with chromoproteins.
Used E. coli 16s rRNA and sfGFP expression modules as inserted DNA fragments.
Applied the method to distinguish Escherichia coli (E. coli) colonies on LB-agar plates.

Main Results:

Rainbow Screening allows visual distinction of correct plasmid candidates by naked eye.
False positive colonies were easily identified on LB-agar plates.
Achieved over 80% for both assembly efficiency and construct accuracy.

Conclusions:

Rainbow Screening offers a simplified, cost-effective, and efficient method for molecular cloning.
This technique eliminates the need for additional genetic manipulations and associated costs.
Rainbow Screening is anticipated to advance molecular cloning methodologies and chromoprotein applications in biotechnology and synthetic biology.