Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

2.1K
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
2.1K
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

388
Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
388
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

350
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
350
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

883
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
883
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

2.1K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
2.1K
Atomic Absorption Spectroscopy: Atomization Methods01:25

Atomic Absorption Spectroscopy: Atomization Methods

418
Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the...
418

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MFAP5<sup>+</sup> synovial fibroblasts drive LOX upregulation to promote osteoarthritis progression.

iScience·2026
Same author

MAO/SA composite coating on magnesium alloy: Corrosion resistance improvement and time-programmed Ca<sup>2 +</sup>/Mg<sup>2+</sup> release for bone repair.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Genome-Wide Analysis and Expression Profiles of <i>AhLOG</i> Gene Family in Peanut (<i>Arachis hypogaea</i> L.).

International journal of molecular sciences·2026
Same author

Learning like a radiologist: a medical vision-language model for radiological image analysis via curriculum learning.

NPJ digital medicine·2026
Same author

Large-Scale Stereolithography Precision Manufactured Solid Cone-Jet Emitters for Electrospray Mass Spectrometry.

Analytical chemistry·2026
Same author

Predict neuromuscular performance in human epidural electrical stimulation: phase 1 trial interim results.

Communications medicine·2026

Related Experiment Video

Updated: Jun 28, 2025

Real-Time Void Spot Assay
06:39

Real-Time Void Spot Assay

Published on: February 10, 2023

2.0K

Time-averaged atomic volume spectrum: locating and identifying vacancies.

YongQuan Wu1, Hao Wang1, JiaHao Fu1

  • 1State Key Laboratory of Advanced Special Steel & Shanghai Key Laboratory of Advanced Ferrometallurgy & School of Materials Science and Engineering, Shanghai University, Shanghai, 200444, China. yqwu@shu.edu.cn.

Materials Horizons
|April 16, 2024
PubMed
Summary

This study introduces a novel method to accurately locate and characterize vacancies in materials by analyzing their surrounding atomic cages. This approach overcomes challenges posed by thermal vibrations and vacancy migration, enabling detailed defect mapping.

More Related Videos

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

6.0K
Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation
09:53

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation

Published on: October 30, 2012

13.0K

Related Experiment Videos

Last Updated: Jun 28, 2025

Real-Time Void Spot Assay
06:39

Real-Time Void Spot Assay

Published on: February 10, 2023

2.0K
Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

6.0K
Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation
09:53

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation

Published on: October 30, 2012

13.0K

Area of Science:

  • Materials Science
  • Condensed Matter Physics
  • Computational Materials Science

Background:

  • Vacancies significantly influence material properties but are difficult to precisely locate due to thermal vibrations and migration.
  • Existing methods lack the accuracy and flexibility needed for comprehensive vacancy analysis, hindering scientific advancement.

Purpose of the Study:

  • To develop an accurate and flexible method for locating, identifying, and characterizing vacancies in materials.
  • To overcome the limitations of previous techniques in dealing with thermal vibrations and vacancy mobility.

Main Methods:

  • Introduced a new strategy focusing on the atomic cage surrounding vacancies instead of the vacancies themselves.
  • Utilized a time-averaged atomic volume spectrum (TAVS) to identify atoms, effectively denoising thermal vibrations and preventing vacancy migration.
  • Applied the TAVS method to quenched and annealed face-centered cubic (FCC) aluminum.

Main Results:

  • Successfully generated panoramic maps of spontaneously trapped defects in FCC Al for the first time.
  • Obtained instantaneous images of steady trapping processes, revealing detailed characteristics of each vacancy (location, dimension, volume, morphology).
  • Accurately determined aggregate statistical data, including vacancy amount and concentration.

Conclusions:

  • The TAVS method provides a robust means for locating, identifying, and characterizing vacancies, offering unprecedented detail.
  • The study revealed novel phenomena in defect behavior within FCC Al, opening avenues for future research.
  • The TAVS method is adaptable and can be extended to various other material systems beyond FCC Al.