Bioactive properties and gut microbiota modulation by date seed polysaccharides extracted using ultrasound-assisted...

Area of Science:

• Food Science
• Biochemistry
• Microbiology

Background:

• Date seeds are a rich source of polysaccharides.
• Valorization of agricultural by-products like date seeds is crucial for sustainability.

Purpose of the Study:

• To extract and characterize polysaccharides from date seeds (UPS).
• To evaluate the bioactivity, prebiotic potential, and gut microbiome modulation of UPS.

Main Methods:

• Ultrasound-assisted deep eutectic solvent extraction for UPS isolation.
• Comprehensive characterization including chemical composition and bioactivity assays (antioxidant, antimicrobial, enzyme inhibition).
• In vitro evaluation of prebiotic properties and gut microbiota fermentation.

Main Results:

• UPS exhibited significant antioxidant and antimicrobial activities against tested pathogens.
• UPS demonstrated notable inhibition of α-glycosidase, α-amylase, and ACE, and reduced cancer cell viability.
• UPS promoted beneficial gut microbiota growth and short-chain fatty acid (SCFA) production.

Conclusions:

• Date seed polysaccharides are a promising source of bioactive compounds.
• UPS possess antioxidant, antimicrobial, enzyme inhibitory, and prebiotic properties.
• UPS can be utilized as functional ingredients for food and potentially pharmaceutical applications.