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  11. Abscisic Acid Signaling Through Lancl2 And Pparγ Induces Activation Of P38mapk Resulting In Dormancy Of Prostate Cancer Metastatic Cells

Abscisic acid signaling through LANCL2 and PPARγ induces activation of p38MAPK resulting in dormancy of prostate cancer metastatic cells

Keshab Raj Parajuli1, Younghun Jung2, Russell S Taichman1

  • 1Department of Periodontology, University of Alabama at Birmingham School of Dentistry, Birmingham, AL 35294, USA.

Oncology Reports
|April 16, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Abscisic acid (ABA) induces dormancy in prostate cancer (PCa) cells by signaling through LANCL2 and PPARγ. This suggests ABA as a potential therapeutic target for managing metastatic PCa growth.

Area of Science:

  • Oncology
  • Molecular Biology
  • Endocrinology

Background:

  • Prostate cancer (PCa) frequently metastasizes to bone, with disseminated tumor cells entering dormancy.
  • Abscisic acid (ABA), a plant stress hormone, is also found in mammals and linked to human diseases.
  • Understanding mechanisms of tumor dormancy is crucial for treating metastatic PCa.

Purpose of the Study:

  • To investigate the role of ABA in regulating PCa cell dormancy.
  • To elucidate the signaling pathway involving lanthionine synthetase C-like protein 2 (LANCL2) and peroxisome proliferator activated receptor γ (PPARγ) in ABA-mediated dormancy.

Main Methods:

  • Utilized human PCa cell lines with targeted gene knockdown (KD) of LANCL2 and PPARγ.
  • Employed western blotting, quantitative PCR, cell proliferation, migration, invasion, and soft agar assays.
Keywords:
abscisic aciddisseminated tumor cellsdormancylanthionine synthetase C‑like protein 2

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  • Conducted co-culture assays with bone marrow stromal cells.

    • Main Results:

    • ABA treatment increased expression of cell cycle inhibitors (p21, p27, p16) and inhibited PCa cell viability, migration, invasion, and colony formation.
    • ABA induced p38MAPK activation and NR2F1 signaling.
    • Knockdown of LANCL2 or PPARγ abolished ABA's effects on PCa cells.

    Conclusions:

    • ABA induces dormancy in PCa cell lines via the LANCL2 and PPARγ signaling pathway.
    • ABA represents a potential therapeutic strategy for managing metastatic PCa.
    • LANCL2 and PPARγ are potential novel targets for controlling metastatic PCa growth.
    peroxisome proliferator‑activated receptor γ
    prostate cancer