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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Gene-Environment Interactions01:20

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Gene expression is a dynamic process that is significantly influenced by environmental factors. This interaction underlies the complex nature of biological development and the phenotypic differences observed among individuals, even among those with identical genetic makeups. Factors such as radiation, temperature, behavior, nutrition, and stress play pivotal roles in determining how genes are expressed. The concept of the reaction range is central to understanding this interaction. It posits...
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
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Updated: Jun 28, 2025

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
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Environmental DNA: The next chapter.

Rosetta Blackman1,2, Marjorie Couton1,2, François Keck1,2

  • 1Department of Aquatic Ecology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, Dübendorf, Switzerland.

Molecular Ecology
|April 16, 2024
PubMed
Summary
This summary is machine-generated.

Environmental DNA (eDNA) has revolutionized biodiversity research, enabling widespread species detection. This review assesses eDNA

Keywords:
biomonitoringeDNA behaviourprimersquantificationreference databasesampling

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Area of Science:

  • Ecology
  • Biodiversity Science
  • Molecular Biology

Background:

  • Environmental DNA (eDNA) analysis has become a cornerstone of modern ecological and biodiversity research.
  • Initial applications focused on population dynamics, but the scope has expanded significantly to include metabarcoding for broad biodiversity assessments.

Observation:

  • A decade after landmark expectations, eDNA research has seen substantial success in sample collection, primer development, biomonitoring, quantification, DNA behavior studies, and reference database creation.
  • Despite successes, certain expectations remain unmet, necessitating further research and development.

Findings:

  • The field has progressed rapidly, uncovering previously unknown biodiversity across diverse ecosystems and taxonomic groups.
  • A comprehensive review of literature and user surveys highlights both achievements and areas requiring focused research efforts.

Implications:

  • The future of eDNA is promising, with ongoing technical advancements.
  • Interdisciplinary collaboration and standardized best practices are crucial for realizing the full potential of eDNA in biodiversity conservation and research.