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Updated: Jun 28, 2025

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
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Cytochrome P450 Mining for Bufadienolide Diversification

Xiaolai Lei1, Xiaozheng Wang1, Weiliang Xiong1

  • 1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory on Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

ACS Chemical Biology
|April 16, 2024
PubMed
Summary

Researchers identified toad cytochrome P450 enzymes that produce novel bufadienolides with antitumor activity. These enzymes enable efficient, site-specific synthesis of valuable steroid compounds.

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