Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Population pharmacokinetics of dalbavancin: external validation, model averaging, and implications for precision dosing in prolonged therapy.

Antimicrobial agents and chemotherapy·2026
Same author

Causal inference and digital twins: a roadmap for the future of clinical trials.

NPJ digital medicine·2026
Same author

Human serum albumin profiling by top-down analysis enables multi-class liver fibrosis staging: a cross-platform validation study.

Scientific reports·2026
Same author

Monte Carlo simulations identify suboptimal PK/PD target attainment with standard maribavir dosing.

The Journal of antimicrobial chemotherapy·2026
Same author

Learned spatially varying microscopy model with adaptive point spread functions.

Optics express·2026
Same author

Intragraft clonal expansion of cytotoxic CD8<sup>+</sup> and CD4<sup>+</sup> T cells in antibody-mediated kidney transplant rejection.

Kidney international·2026

Related Experiment Video

Updated: Jun 28, 2025

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
00:07

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

8.0K

High Precision Ping-Pong Auto-Zeroed Lock-in Fluorescence Photometry Sensor.

Vahid Khojasteh Lazarjan, Marie-Eve Crochetiere, Mehdi Noormohammadi Khiarak

    IEEE Transactions on Biomedical Circuits and Systems
    |April 16, 2024
    PubMed
    Summary

    This study introduces a high-precision CMOS fluorescence sensor with a novel lock-in amplification scheme for real-time in vitro biomarker detection. The portable device demonstrates high sensitivity in detecting cell cultures and solutions.

    More Related Videos

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    11.3K
    Fluorescence Imaging with One-nanometer Accuracy FIONA
    11:56

    Fluorescence Imaging with One-nanometer Accuracy FIONA

    Published on: September 26, 2014

    17.7K

    Related Experiment Videos

    Last Updated: Jun 28, 2025

    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
    00:07

    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

    Published on: August 22, 2019

    8.0K
    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    11.3K
    Fluorescence Imaging with One-nanometer Accuracy FIONA
    11:56

    Fluorescence Imaging with One-nanometer Accuracy FIONA

    Published on: September 26, 2014

    17.7K

    Area of Science:

    • Biomedical Engineering
    • Microelectronic Systems
    • Optical Sensing

    Background:

    • Accurate and portable fluorescence detection is crucial for in vitro diagnostics.
    • Existing methods often face challenges with sensitivity, power consumption, and real-time analysis.
    • Novel sensor designs are needed to overcome these limitations for ambulatory applications.

    Purpose of the Study:

    • To present a high-precision CMOS fluorescence photometry sensor.
    • To implement a novel lock-in amplification (LIA) scheme using switched-biasing and ping-pong auto-zeroing techniques.
    • To develop a portable, low-power platform for real-time in vitro biomarker detection.

    Main Methods:

    • Fabrication of a CMOS sensor with two photodiodes and a 1 kHz LIA in a 0.18-µm process.
    • Utilizing a differential low-noise amplifier with switched-biasing ping-pong auto-zeroing, automatic phase aligner, PGA, BPF, mixer, and LPF.
    • Encapsulating the sensor in a 3D-printed housing with an LED and fiber optics for excitation and fluorescence retrieval.

    Main Results:

    • The LIA demonstrated a dynamic reserve of 42 dB, retrieving noisy signals with 0.7 mW power consumption from a 1.8 V supply.
    • The sensor detected incident light power ranging from 8 nW to 24 µW.
    • Experiments showed a sensitivity of 1:100 k for bead solutions and successful detection of varying NIH3T3 mouse cell densities in vitro.

    Conclusions:

    • The developed CMOS fluorescence sensor with a novel LIA scheme offers high precision and sensitivity for in vitro diagnostics.
    • The portable, low-power ambulatory platform enables real-time biomarker detection.
    • The prototype's performance in detecting cell cultures validates its potential for point-of-care applications.