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Expression patterns of mechanosensitive ion channel PIEZOs in irreversible pulpitis
Wenying Yang1, Lu Lin1, Shucheng Hu1
1Department of Stomatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 160 Pujian Road, Shanghai, 200127, China.
BMC Oral Health
|April 16, 2024
Summary
Mechanosensitive ion channels PIEZO1 and PIEZO2 are linked to irreversible pulpitis. PIEZO1 is upregulated, while PIEZO2 is downregulated, suggesting roles in pulp inflammation and pain.
Area of Science:
- Biomedical Science
- Molecular Biology
- Dental Research
Background:
- Mechanosensitive ion channels, known as PIEZOs, are implicated in inflammation and pain signaling.
- Understanding PIEZO expression in dental pulp is crucial for investigating conditions like irreversible pulpitis.
Purpose of the Study:
- To investigate the expression patterns of PIEZO1 and PIEZO2 in irreversible pulpitis.
- To explore the correlation between PIEZO expression and clinical pain levels or inflammatory markers.
Main Methods:
- Pulp tissues from normal and irreversible pulpitis patients were analyzed for PIEZO mRNA and protein expression.
- Real-time PCR, immunoblotting, immunohistochemistry, and analysis of public datasets (GSE77459) were employed.
- Correlations with inflammatory markers, pain markers, and clinical pain assessments were evaluated.
Main Results:
- PIEZO1 mRNA levels were significantly increased in irreversible pulpitis compared to normal pulp.
- PIEZO2 mRNA levels were significantly decreased in irreversible pulpitis.
- PIEZO1 correlated with inflammatory markers, while PIEZO2 correlated with pain markers and clinical pain levels.
Conclusions:
- PIEZO1 is upregulated and PIEZO2 is downregulated in irreversible pulpitis.
- PIEZO channels are potentially involved in the progression of inflammation and pain in irreversible pulpitis.
- Targeting PIEZOs may offer novel therapeutic strategies for irreversible pulpitis.
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