Related Experiment Video
Updated: Jun 28, 2025
10:33
An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue
Published on: February 3, 2023
1.3K
An exosome mRNA-related gene risk model to evaluate the tumor microenvironment and predict prognosis in
Zhonghai Du1, Xiuchen Han2, Liping Zhu3
1Department of Medical Oncology, Weifang Hospital of Traditional Chinese Medicine, Weifang, Shandong Province, China.
BMC Medical Genomics
|April 16, 2024
Summary
This study developed a prognostic model using exosome mRNA profiles to predict outcomes in hepatocellular carcinoma (HCC). This exosome mRNA signature aids in risk stratification and understanding the tumor microenvironment (TME) in HCC.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- The role of exosomes in the tumor microenvironment (TME) of hepatocellular carcinoma (HCC) is not well understood.
- This study investigates the impact of exosome mRNA expression on the HCC TME.
Purpose of the Study:
- To establish an exosome mRNA-related risk score model for prognostic value in HCC.
- To explore the functional pathways and immune cell infiltration associated with exosome mRNA signatures in HCC.
Main Methods:
- Utilized exosome mRNA profiles from exoRBase and RNA sequencing data from TCGA and ICGC for HCC patients.
- Developed and validated a prognostic 4-gene signature (DYNC1H1, PRKDC, CCDC88A, ADAMTS5) and a prognostic nomogram.
- Performed functional enrichment analysis and assessed immune cell abundance in the TME using CIBERSORT.
Main Results:
- A validated 4-gene exosome mRNA signature demonstrated prognostic value for HCC.
- The identified genes are implicated in extracellular matrix interactions and the PI3K-Akt signaling pathway.
- Gene expression positively correlated with immune cell infiltration in the HCC tumor microenvironment.
Conclusions:
- An exosome mRNA-related risk model can be effectively established for hepatocellular carcinoma.
- This model highlights the functional importance of exosome mRNA in HCC prognosis and risk stratification.
Related Concept Videos
01:36Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
02:17The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K